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Stoichiometry and current kinetics of KCNQ1-KCNE1 ion channel complex

Research Project

Project/Area Number 24590292
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field General physiology
Research InstitutionNational Institute for Physiological Sciences

Principal Investigator

NAKAJO Koichi  生理学研究所, 分子生理研究系, 助教 (80390699)

Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2012: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Keywordsイオンチャネル / カリウムチャネル / 蛍光イメージング / KCNQ1 / KCNE1 / 全反射蛍光顕微鏡 / Kv4 / FRET / KCNQチャネル / KCNE / 不整脈 / QT延長症候群 / 電位依存性 / 複合体
Outline of Final Research Achievements

I investigated how the expression level of KCNE1 affected the slow current kinetics of a cardiac voltage-gated potassium channel KCNQ1. I found that the more binding of KCNE1 to KCNQ1, the less activity KCNQ1 showed. I also found that the longer the distance between the two subunits, the slower the activation kinetics became. In another cardiac voltage-gated potassium channel Kv4, the current kinetics was also dependent on the expression level of its auxiliary subunits KChIP4 and DPP10. The expression levels of auxiliary subunits for ion channels could be a key factor in cardiac excitability.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • 2012 Research-status Report
  • Research Products

    (15 results)

All 2015 2014 2013 Other

All Journal Article (4 results) (of which Peer Reviewed: 4 results,  Acknowledgement Compliant: 3 results) Presentation (9 results) (of which Invited: 2 results) Remarks (2 results)

  • [Journal Article] KCNQ1 channel modulation by KCNE proteins via the voltage-sensing domain2015

    • Author(s)
      Koichi Nakajo, Yoshihiro Kubo
    • Journal Title

      Journal of Physiology

      Volume: in press Issue: 12 Pages: 2617-2625

    • DOI

      10.1113/jphysiol.2014.287672

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Steric hindrance between S4 and S5 of the KCNQ1/KCNE1 channel hampers pore opening2014

    • Author(s)
      Koichi Nakajo, Yoshihiro Kubo
    • Journal Title

      Nature Communications

      Volume: 5 Issue: 1 Pages: 4100-4100

    • DOI

      10.1038/ncomms5100

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] The stoichiometry and biophysical properties of the Kv4 potassium channel complex with K+ channel-interacting protein (KChIP) subunits are variable, depending on the relative expression level2014

    • Author(s)
      Masahiro Kitazawa, Yoshihiro Kubo, Koichi Nakajo
    • Journal Title

      Journal of Biological Chemistry

      Volume: 289 Issue: 25 Pages: 17597-17609

    • DOI

      10.1074/jbc.m114.563452

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] 電位依存性カリウムチャネルKCNQ1の複合体ストイキオメトリーと機能制御機構2013

    • Author(s)
      中條浩一、久保義弘
    • Journal Title

      生物物理

      Volume: 53 Pages: 313-316

    • NAID

      10031203608

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Presentation] QT短縮症候群を起こす変異を持ったKCNQ1チャネルの電位センサーの動きの変化2015

    • Author(s)
      中條浩一、久保義弘
    • Organizer
      第120回日本解剖学会総会・全国学術集会 第92回日本生理学会大会
    • Place of Presentation
      神戸国際会議場・展示場(兵庫県神戸市)
    • Year and Date
      2015-03-21
    • Related Report
      2014 Annual Research Report
  • [Presentation] hERGチャネルの遅い脱活性化に関与するEAGドメイン、S4-S5リンカー、およびCリンカーの相互作用の解析2015

    • Author(s)
      粂慎一郎、中條浩一、久保義弘
    • Organizer
      第120回日本解剖学会総会・全国学術集会 第92回日本生理学会大会
    • Place of Presentation
      神戸国際会議場・展示場(兵庫県神戸市)
    • Year and Date
      2015-03-21
    • Related Report
      2014 Annual Research Report
  • [Presentation] Kv4.2/DPP10複合体は4:2チャネルを形成しやすい2015

    • Author(s)
      北沢和寛、中條浩一、久保義弘
    • Organizer
      第120回日本解剖学会総会・全国学術集会 第92回日本生理学会大会
    • Place of Presentation
      神戸国際会議場・展示場(兵庫県神戸市)
    • Year and Date
      2015-03-21
    • Related Report
      2014 Annual Research Report
  • [Presentation] KCNQ1/KCNE1チャネルを開きにくくしている分子メカニズム2014

    • Author(s)
      中條浩一、久保義弘
    • Organizer
      中部日本生理学会
    • Place of Presentation
      名古屋市立大学(愛知県名古屋市)
    • Year and Date
      2014-11-08
    • Related Report
      2014 Annual Research Report
  • [Presentation] Dynamic aspects of the subunit stoichiometry of the KCNQ1/KCNE channel complex

    • Author(s)
      Koichi Nakajo, Yoshihiro Kubo
    • Organizer
      第37回国際生理学会議(IUPS2013)
    • Place of Presentation
      Birmingham, UK
    • Related Report
      2013 Research-status Report
    • Invited
  • [Presentation] A pair of phenylalanine residues on the S4 and S5 segments create a physical and energy barrier for the voltage sensor in KCNQ1/KCNE1 channel

    • Author(s)
      Koichi Nakajo, Yoshihiro Kubo
    • Organizer
      Biophysical Society 58th Annual Meeting
    • Place of Presentation
      San Francisco, USA
    • Related Report
      2013 Research-status Report
  • [Presentation] S4およびS5セグメントのフェニルアラニン残基がつくる電位センサードメインに対する物理的な障壁がKCNQ1/KCNE1チャネルのゲーティングを遅くする

    • Author(s)
      中條浩一、久保義弘
    • Organizer
      第91回日本生理学会
    • Place of Presentation
      鹿児島大学(鹿児島)
    • Related Report
      2013 Research-status Report
  • [Presentation] イオンチャネル修飾サブユニットの追加による生理機能進化

    • Author(s)
      中條浩一
    • Organizer
      第14回日本進化学会
    • Place of Presentation
      首都大学東京(東京都)
    • Related Report
      2012 Research-status Report
    • Invited
  • [Presentation] 電位センサードメインを介したKCNQチャネルのゲーティング修飾機構

    • Author(s)
      中條浩一、久保義弘
    • Organizer
      第90回日本生理学会大会
    • Place of Presentation
      タワーホール船堀(東京都)
    • Related Report
      2012 Research-status Report
  • [Remarks] 日本生理学会ホームページ サイエンストピックス

    • URL

      http://physiology.jp/science-topic/10510/

    • Related Report
      2014 Annual Research Report
  • [Remarks] 生理学研究所ホームページ プレスリリース

    • URL

      http://www.nips.ac.jp/release/2014/06/kcnq1kcne1.html

    • Related Report
      2014 Annual Research Report

URL: 

Published: 2013-05-31   Modified: 2019-07-29  

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