Stoichiometry and current kinetics of KCNQ1-KCNE1 ion channel complex
Project/Area Number |
24590292
|
Research Category |
Grant-in-Aid for Scientific Research (C)
|
Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
General physiology
|
Research Institution | National Institute for Physiological Sciences |
Principal Investigator |
NAKAJO Koichi 生理学研究所, 分子生理研究系, 助教 (80390699)
|
Project Period (FY) |
2012-04-01 – 2015-03-31
|
Project Status |
Completed (Fiscal Year 2014)
|
Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2012: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
|
Keywords | イオンチャネル / カリウムチャネル / 蛍光イメージング / KCNQ1 / KCNE1 / 全反射蛍光顕微鏡 / Kv4 / FRET / KCNQチャネル / KCNE / 不整脈 / QT延長症候群 / 電位依存性 / 複合体 |
Outline of Final Research Achievements |
I investigated how the expression level of KCNE1 affected the slow current kinetics of a cardiac voltage-gated potassium channel KCNQ1. I found that the more binding of KCNE1 to KCNQ1, the less activity KCNQ1 showed. I also found that the longer the distance between the two subunits, the slower the activation kinetics became. In another cardiac voltage-gated potassium channel Kv4, the current kinetics was also dependent on the expression level of its auxiliary subunits KChIP4 and DPP10. The expression levels of auxiliary subunits for ion channels could be a key factor in cardiac excitability.
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Report
(4 results)
Research Products
(15 results)