Identification of master molecules in signal transduction of canceled cells using TAT protein
Project/Area Number |
24590301
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Environmental physiology (including Physical medicine and Nutritional physiology)
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Research Institution | Yamaguchi University |
Principal Investigator |
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Co-Investigator(Kenkyū-buntansha) |
TAKAMURA Ayumi 鳥取大学, 医学部, 講師 (90508368)
|
Project Period (FY) |
2012-04-01 – 2016-03-31
|
Project Status |
Completed (Fiscal Year 2015)
|
Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
|
Keywords | がん / 蛋白精製 / エイズ / 乳癌 / 細胞培養 / 分散 / シグナル伝達 / リン酸化 / タンパク質導入 / 陰イオン交換カラム / 精製 / 蛋白導入 / 癌 / シグナル経路 |
Outline of Final Research Achievements |
In this study, we have established a new screening method based on protein introduction method using the TAT protein to identify the proteins involved in carcinogenesis at the cellular level. In three Peak were isolated from estrogen-dependent breast cancer cells MCF-7, and mitochondrial activity in the PeakC was decrease. Based on the analysis by mass spectrometry, a protein in the Peak C was identified as Histone H4, which may inhibit mitochondrial activity. The future is going examined whether to suppress how to mitochondrial activity is Histone H4. The activities were disappeared in PeakA and B during protein purification. Dephosphorylations of the proteins could be caused during the protein separation. Therefore, increases of protein contents and assay sensitivity would be requested for the next experiments.
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Report
(5 results)
Research Products
(24 results)
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[Journal Article] Enhanced sialylation of a human chimeric IgG1 variant produced in human and rodent cell lines2016
Author(s)
Mimura, Y.,Kelly, R.M.,Unwin, L., Albrecht, S., Jefferis, R., Goodall, M., Yoichi Mizukami, Y., Mimura-Kimura, Y., Matsumoto, T., Ueoka, H., Pauline M Rudd, P.M.
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Journal Title
J. Immunol Methods,
Volume: 428
Pages: 30-36
Related Report
Peer Reviewed / Int'l Joint Research
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[Journal Article] The combination of insulin-like growth factor 1and erythropoietin protects against ischemic spinal cord injury in rabbits2015
Author(s)
Utada, K., Ishida, K., Tohyama, S., Urushima, Y., Mizukami, Y., Yamashita, A., Uchida, M., Matsumoto, M.
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Journal Title
J. Anesthesia
Volume: 29
Issue: 5
Pages: 741-748
DOI
Related Report
Peer Reviewed
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[Journal Article] Optimizing high-resolution melting analysis for the detection of mutations of GPR30/GPER-1 in breast cancer2012
Author(s)
Aihara, M., Yamamoto, S., Nishioka, H., Hamano, K., Oka, M., Mizukami, Y.
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Journal Title
Gene
Volume: (in press)
Issue: 2
Pages: 95-105
DOI
Related Report
Peer Reviewed
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