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Serial estimation of behavioral capacity and brain damages of rat models of neonatal hyperthermic hypoxic-ischemic encephalopathy

Research Project

Project/Area Number 24590305
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Environmental physiology (including Physical medicine and Nutritional physiology)
Research InstitutionOsaka Electro-Communication University

Principal Investigator

HOSONO Takayoshi  大阪電気通信大学, 医療福祉工学部, 教授 (60294104)

Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Keywords新生児低酸素虚血脳障害 / 高体温 / ラット / 運動能力 / 知能 / 時系列 / 組織染色
Outline of Final Research Achievements

In order to establish a rat model of human neonatal hyperthermic hypoxic-ischemic encephalopathy (HIE), a left carotid artery of 7-day-old Wistar rat was ligated under anesthesia. After 1-h recovery, the rat was exposed to 8% oxygen at an ambient temperature of 40 degree for 15 min. After growth, retention time of rats to avoid disgust shocks was measured by employing step-down passive avoidance test. Duration of rats to stay on rotating rod was also estimated by Rotarod test. After the behavioral tests, the rat brains were removed under deep anesthesia and the largest width of cerebrum hemispheres was measured. The area of CA1 hippocampus was also measured using preparation specimen of the brain.
The results of the behavioral tests were significantly worse in the HIE models than in the control. The largest width of cerebrum hemispheres and the CA1 area of the HIE models were smaller than those of the control. The neonatal hyperthermic HIE rat models were successfully established.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • 2012 Research-status Report
  • Research Products

    (9 results)

All 2015 2014 2013 2012

All Journal Article (1 results) (of which Peer Reviewed: 1 results,  Acknowledgement Compliant: 1 results) Presentation (8 results)

  • [Journal Article] Effects of 3-h hypothermia after neonatal hyperthermic hypoxic-ischemic encephalopathy in rat models on behavioral prognosis, and anatomical and histological features after growth.2015

    • Author(s)
      Nishimura Y, Hosono T
    • Journal Title

      Journal of Maternal Fetal & Neonatal Medicine

      Volume: 未定

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Presentation] ラット新生仔高体温低酸素虚血性脳症に対する成長後の学習能力と脳低温療法の効果2015

    • Author(s)
      西村友香子, 細野剛良
    • Organizer
      第67回日本産科婦人科学会学術講演会
    • Place of Presentation
      横浜市(パシフィコ横浜)
    • Year and Date
      2015-04-09 – 2015-04-12
    • Related Report
      2014 Annual Research Report
  • [Presentation] Motor performance of rat models after neonatal hypoxic ischemic encephalopathy with different periods of brain insult assessed using Rotarod and traction tests.2014

    • Author(s)
      Hosono T, Nishimura Y, Ishida M, Arakawa M, Nakamoto M, Akasaka K.
    • Organizer
      The 20th World Congress on Controversies in Obstetrics, Gynecology & Infertility
    • Place of Presentation
      Paris (Marriott Rive Gauche Hotel & Conference Centre), France
    • Year and Date
      2014-12-04 – 2014-12-07
    • Related Report
      2014 Annual Research Report
  • [Presentation] Effect of hyperthermia on motor function of adult rats after neonatal hypoxic-ischemic encephalopathy2014

    • Author(s)
      Nishimura Y, Minato K, Hosono T
    • Organizer
      FENS Forum 2014
    • Place of Presentation
      Milano (Milano Congressi), Italy
    • Year and Date
      2014-07-05 – 2014-07-09
    • Related Report
      2014 Annual Research Report
  • [Presentation] Effects of duration of hyperthermic hypoxia on neonatal rats based on motor function and immunohistochemical staining after growth2014

    • Author(s)
      Hosono T, Nishimura Y, Arakawa M, Nakamoto M, Akasaka K, Minato K
    • Organizer
      FENS Forum 2014
    • Place of Presentation
      Milano (Milano Congressi), Italy
    • Year and Date
      2014-07-05 – 2014-07-09
    • Related Report
      2014 Annual Research Report
  • [Presentation] Effect of hyperthermia on adult rats after neonatal hypoxic-ischemic encephalopathy on learning ability2014

    • Author(s)
      西村友香子、細野剛良
    • Organizer
      第91回日本生理学会大会
    • Place of Presentation
      鹿児島大学、鹿児島市
    • Related Report
      2013 Research-status Report
  • [Presentation] Effects of duration of hyperthermic hypoxia on neonatal rats assessed after growth by behavior2013

    • Author(s)
      Hosono T, Yamamoto M, Igi C, Akasaka K, Arakawa M, Nakamoto M, Minato K.
    • Organizer
      IUPS 2013 (37th International Congress of Physiological Sciences)
    • Place of Presentation
      International Convention Centre, Birmingham, UK
    • Related Report
      2013 Research-status Report
  • [Presentation] マウス行動性体温調節の環境温度による変化の二者択一式床面温度選択装置による評価2013

    • Author(s)
      細野剛良、井関多久美、秦朋裕、赤坂佳祐、山本愛美、伊木千尋、荒川茉由、中本実希、西村友香子
    • Organizer
      第90回生理学会大会
    • Place of Presentation
      タワーホール船堀, 東京
    • Related Report
      2012 Research-status Report
  • [Presentation] Behavioral effects during and after growth of rats administered betamethasone in immaturity2012

    • Author(s)
      Takayoshi Hosono, Chiaki Nitta, Megumi Yamamoto, Chihiro Igi, and Kenji Minato
    • Organizer
      The XX FIGO World Congress of Gynecology & Obstetrics
    • Place of Presentation
      Fiera di Roma, Roma, Italy
    • Related Report
      2012 Research-status Report

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Published: 2013-05-31   Modified: 2019-07-29  

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