Elucidation of spatio-temporal regulation of the intracellular configuration of GPCR-signaling complex
| Project/Area Number |
24590311
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
General pharmacology
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| Research Institution | Tohoku University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
Sukegawa JUN 東北大学, 医学系研究科, 客員教授 (30187687)
SATO Takeya 東北大学, 医学系研究科, 准教授 (10312696)
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| Project Period (FY) |
2012-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | 受容体 / チャネル / 輸送系 / シグナル情報伝達系 / GHRHR / PICK1 / LNCaP / ガン細胞 / 細胞増殖 / ERK情報伝達系 / シグナル情報伝達 |
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| Outline of Final Research Achievements |
G-protein coupled receptors are located at the cell surface to transduce the extracellular information into the cells by converting it as a second messenger. Following transducing the signal, GPCR are sequestrated from the cellular surface by interacting with many cytoplasmic proteins. These GPCR-interacting proteins (GIPs) are crucial for the regulation of the intracellular localization and the signaling of GPCR, however, the mechanisms of the regulation between the GIPs and GPCR remained unresolved. We have focused on one of the GPCR, growth hormone releasing hormone receptors and its interacting protein, PICK1. We have found that PICK1 regulated the cell surface expression and the intracellular signaling of GHRHR. Furthermore, the interaction of PICK1 with GHRHR could regulate the cellular proliferation of human prostate adenocarcinoma cells, LNCap.
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Report
(5 results)
Research Products
(33 results)
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[Journal Article] Modified autonomic regulation in mice mutated in the ß4 subunit of the lh/lh calcium channel.2015
Author(s)
Murakami M, Suzuki T, Wu TW, Kuwasako K, Takahashi E, Watanabe H, Murakami AM, Miyoshi I, Yanagisawa T, Sasano H, Ono K, Ohba T
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Journal Title
Biochem Biophys Res Commun
Volume: pii: S0006-291X(15)
Issue: 2
Pages: 200-205
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Mitochonic Acid 5 (MA-5), a Derivative of the Plant Hormone Indole-3-Acetic Acid, Improves Survival of Fibroblasts from Patients with Mitochondrial Diseases2015
Author(s)
Suzuki T, Yamaguchi H, Kikusato M, Matsuhashi T, Matsuo A, Sato T, Oba Y, Watanabe S, Minaki D, Saigusa D, Shimbo H, Mori N, Mishima E, Shima H, Akiyama Y, Takeuchi Y, Yuri A, Kikuchi K, Toyohara T, Suzuki C, Kohzuki M, Anzai J, Mano N, Kure S, Yanagisawa T, Tomioka Y, Toyomizu M, Ito S, Osaka H, Hayashi K, Abe T
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Journal Title
The Tohoku Journal of Experimental Medicine
Volume: 236
Issue: 3
Pages: 225-232
DOI
NAID
ISSN
0040-8727, 1349-3329
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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[Journal Article] Involvement of the histamine H1 receptor in the regulation of sympathetic nerve activity.2015
Author(s)
Murakami M1, Yoshikawa T2, Nakamura T2, Ohba T3, Matsuzaki Y4, Sawamura D4, Kuwasako K5, Yanagisawa T6, Ono K3, Nakaji S7, Yanai K2.
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Journal Title
Biochem Biophys Res Commun.
Volume: 458
Issue: 4
Pages: 584-589
DOI
Related Report
Peer Reviewed
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[Journal Article] Interaction of PICK1 with C-Terminus of Growth Hormone–Releasing Hormone Receptor (GHRHR) Modulates Trafficking and Signal Transduction of Human GHRHR2013
Author(s)
Katsushima Y, Sato T., Yamada C., Ito M., Suzuki Y., Ogawa E., Sukegawa I., Sukegawa J., Fukunaga K., Yanagisawa T.
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Journal Title
Journal of Pharmacological Sciences
Volume: 122
Issue: 3
Pages: 193-204
DOI
NAID
ISSN
1347-8613, 1347-8648
Related Report
Peer Reviewed
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