Development of demembranated cell model suitable for analyzing myosin-sliding

• Project/Area Number: 24590312
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: General pharmacology
• Research Institution: Gunma Prefectural College of Health Sciences
• Principal Investigator: ISHIKAWA RYOKI 群馬県立県民健康科学大学, 看護学部, 教授 (20212863)
• Co-Investigator(Kenkyū-buntansha): NAKAMURA Akio 群馬大学, 大学院医学系研究科, 講師 (30282388) ; HOMDA Hajime 長岡技術科学大学, 工学部, 准教授 (20192742)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: アクチン / ミオシン

Outline of Final Research Achievements

We constructed a flow-chamber by using coverslip on which cultured cells had been grown, and cells were treated with surfactants in the presence of high concentration of rhodamine-phalloidin. After changing solutions several times, actin structures remained unchanged. Furthermore, introduction of FITC-labeled myosin II into flow-chamber revealed that it bound to actin structures. Because our system does not contain fixation process, it may be suitable for analyzing myosin-sliding on higher organizations of actin.

Research Products

• [Journal Article] Identification of Alkylbenzene Sulfonate Surfactants Leaching from an Acrylonitrile Butadiene Rubber as Novel Inhibitors of Calcineurin Activity (2013)
  • Author(s): Ito N, Sibuguchi N, Ishikawa R, Tanaka S, Tokita Y, Nakajima-Shimada J, Hosaka K
  • Journal Title: Bioscience, Biotechnology, and Biochemistry Volume: 77 Issue: 5 Pages: 954-960
  • DOI: 10.1271/bbb.120902
  • NAID: 10031177580
  • ISSN: 0916-8451, 1347-6947
  • Peer Reviewed
• [Journal Article] Identification of alkylbenzene sulfonate surfactants leaching from an acrylonitrile butadiene rubber as novel inhibitors of calcineurin activity. (2013)
  • Author(s): Ito N, Sibuguchi N, Ishikawa R, Tanaka S, Tokita Y, Nakajima-Shimada J, Hosaka K
  • Journal Title: Biosci Biotechnol Biochem Volume: in press
  • Peer Reviewed
• [Journal Article] Down-Regulation of Myosin Light Chain Kinase Expression in Vascular Smooth Muscle Cells Accelerates Cell Proliferation: Requirement of Its Actin-binding Domain for Reversion to Normal Rates (2012)
  • Author(s): Wang HH, Nakamura A, Yoshiyama S, Ishikawa R, Cai N, Ye LH, Takano-Ohmuro H, Kohama K
  • Journal Title: Journal of Pharmacological Sciences Volume: 119 Issue: 1 Pages: 91-96
  • DOI: 10.1254/jphs.11213SC
  • NAID: 10030760488
  • ISSN: 1347-8613, 1347-8648
  • Peer Reviewed
• [Presentation] Modulation of monomer configurations of actin filaments by actin-binding proteins. (2014)
  • Author(s): Monma K., Kobayashi K., Ishikawa R., Honda H.
  • Organizer: 第52回日本生物物理学会年会
  • Place of Presentation: 札幌市
  • Year and Date: 2014-09-27
• [Presentation] Properties of the sliding of myosin on actin filaments decorated with various kinds of actin binding proteins. (2014)
  • Author(s): Ishikawa R
  • Organizer: 6th Special Conference of the International Society for Neurochemistry “Dynamic Changes of Nanostructure in the Brain in the Health and Disease: Cutting Edge of the Technical Innovation"
  • Place of Presentation: Tokyo
  • Year and Date: 2014-09-22
  • Invited