Development of demembranated cell model suitable for analyzing myosin-sliding
| Project/Area Number |
24590312
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
General pharmacology
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| Research Institution | Gunma Prefectural College of Health Sciences |
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Principal Investigator |
ISHIKAWA RYOKI 群馬県立県民健康科学大学, 看護学部, 教授 (20212863)
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| Co-Investigator(Kenkyū-buntansha) |
NAKAMURA Akio 群馬大学, 大学院医学系研究科, 講師 (30282388)
HOMDA Hajime 長岡技術科学大学, 工学部, 准教授 (20192742)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | アクチン / ミオシン |
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| Outline of Final Research Achievements |
We constructed a flow-chamber by using coverslip on which cultured cells had been grown, and cells were treated with surfactants in the presence of high concentration of rhodamine-phalloidin. After changing solutions several times, actin structures remained unchanged. Furthermore, introduction of FITC-labeled myosin II into flow-chamber revealed that it bound to actin structures. Because our system does not contain fixation process, it may be suitable for analyzing myosin-sliding on higher organizations of actin.
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Report
(4 results)
Research Products
(5 results)
