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Development of demembranated cell model suitable for analyzing myosin-sliding

Research Project

Project/Area Number 24590312
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field General pharmacology
Research InstitutionGunma Prefectural College of Health Sciences

Principal Investigator

ISHIKAWA RYOKI  群馬県立県民健康科学大学, 看護学部, 教授 (20212863)

Co-Investigator(Kenkyū-buntansha) NAKAMURA Akio  群馬大学, 大学院医学系研究科, 講師 (30282388)
HOMDA Hajime  長岡技術科学大学, 工学部, 准教授 (20192742)
Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywordsアクチン / ミオシン
Outline of Final Research Achievements

We constructed a flow-chamber by using coverslip on which cultured cells had been grown, and cells were treated with surfactants in the presence of high concentration of rhodamine-phalloidin. After changing solutions several times, actin structures remained unchanged. Furthermore, introduction of FITC-labeled myosin II into flow-chamber revealed that it bound to actin structures. Because our system does not contain fixation process, it may be suitable for analyzing myosin-sliding on higher organizations of actin.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • 2012 Research-status Report
  • Research Products

    (5 results)

All 2014 2013 2012

All Journal Article (3 results) (of which Peer Reviewed: 3 results) Presentation (2 results) (of which Invited: 1 results)

  • [Journal Article] Identification of Alkylbenzene Sulfonate Surfactants Leaching from an Acrylonitrile Butadiene Rubber as Novel Inhibitors of Calcineurin Activity2013

    • Author(s)
      Ito N, Sibuguchi N, Ishikawa R, Tanaka S, Tokita Y, Nakajima-Shimada J, Hosaka K
    • Journal Title

      Bioscience, Biotechnology, and Biochemistry

      Volume: 77 Issue: 5 Pages: 954-960

    • DOI

      10.1271/bbb.120902

    • NAID

      10031177580

    • ISSN
      0916-8451, 1347-6947
    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Identification of alkylbenzene sulfonate surfactants leaching from an acrylonitrile butadiene rubber as novel inhibitors of calcineurin activity.2013

    • Author(s)
      Ito N, Sibuguchi N, Ishikawa R, Tanaka S, Tokita Y, Nakajima-Shimada J, Hosaka K
    • Journal Title

      Biosci Biotechnol Biochem

      Volume: in press

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Down-Regulation of Myosin Light Chain Kinase Expression in Vascular Smooth Muscle Cells Accelerates Cell Proliferation: Requirement of Its Actin-binding Domain for Reversion to Normal Rates2012

    • Author(s)
      Wang HH, Nakamura A, Yoshiyama S, Ishikawa R, Cai N, Ye LH, Takano-Ohmuro H, Kohama K
    • Journal Title

      Journal of Pharmacological Sciences

      Volume: 119 Issue: 1 Pages: 91-96

    • DOI

      10.1254/jphs.11213SC

    • NAID

      10030760488

    • ISSN
      1347-8613, 1347-8648
    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Presentation] Modulation of monomer configurations of actin filaments by actin-binding proteins.2014

    • Author(s)
      Monma K., Kobayashi K., Ishikawa R., Honda H.
    • Organizer
      第52回日本生物物理学会年会
    • Place of Presentation
      札幌市
    • Year and Date
      2014-09-27
    • Related Report
      2014 Annual Research Report
  • [Presentation] Properties of the sliding of myosin on actin filaments decorated with various kinds of actin binding proteins.2014

    • Author(s)
      Ishikawa R
    • Organizer
      6th Special Conference of the International Society for Neurochemistry “Dynamic Changes of Nanostructure in the Brain in the Health and Disease: Cutting Edge of the Technical Innovation"
    • Place of Presentation
      Tokyo
    • Year and Date
      2014-09-22
    • Related Report
      2014 Annual Research Report
    • Invited

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Published: 2013-05-31   Modified: 2019-07-29  

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