| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Outline of Final Research Achievements |
An analysis of synaptic properties at individual synapses in living brain preparations is an essential requirement for the study of regulation mechanism underlying synaptic transmission. The aim of this study is to establish a technical platform for a imaging of synaptically released glutamate with a synapse resolution in slice preparations and in vivo. First, we generated novel fluorescent glutamate probes which show more than 10-fold increase of fluorescence intensity, enabling to visualize synaptically released glutamate with high spatiotemporal resolution. Second, we developed methods for a neuron-specific labeling of glutamate probe. Combined these techniques, we tried imaging of spatiotemporal glutamate dynamics in a mouse cortical slice preparation. In this glutamate imaging, glutamate release was visualized at a dendritic spine, indicating that our glutamate imaging platform is applicable to a imaging of synaptically released glutamate in slice preparations and in vivo.
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