In vivo study of CRELD1 as a novel general gerulator for G-protein coupled receptors

• Project/Area Number: 24590316
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: General pharmacology
• Research Institution: University of Fukui
• Principal Investigator: NISHIMUNE Atsushi 福井大学, 医学部, 助教 (40311310)
• Co-Investigator(Kenkyū-buntansha): MURAMATSU Ikunobu 福井大学, 金沢医科大学, 特命教授 (10111965)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000); Fiscal Year 2014: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2012: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
• Keywords: がん / 薬理学一般

Outline of Final Research Achievements

Our previous in vitro studies indicated that the CRELD1 could be a general regulator for multiple G-protein coupled receptors including adrenaline and/or muscarinic acetylcholine receptors. To obtain experimental evidence to support these observations, we created CRELD1 knock-out mice. At least for the heterozygous mice which carried one null allele for CRELD1, showed no significant receptor amount difference to their wild type litter mates. However, we made remarkable observation that these apparently healthy CRELD1 knock-out heterozygous animals developed later terrible maxillary sinus carcinoma at anomalous high frequency. Previous studies identified that 3p25, where human CRELD1 exists , as a candidate locus for the maxillary carcinoma. Although most researchers hypothesize several other tumor suppressor genes as powerful candidate genes, CRELD1 might be a genuine causative gene for the carcinoma. This study highlighted a potential importance of CRELD1 as a disease related gene.

Research Products

• [Journal Article] Radiological impact of the TEPCO's Fukushima Dai-ichi Nuclear Power Plant accident on benthic invertebrates off Fukushima Prefecture (2014)
  • Author(s): Sohtome, T., Wada, T., Mizuno, T., Nemoto, Y., Igarashi, S., Nishimune, A., Aono, T., Ito, Y., Kanda, J., Ishimaru, T.
  • Journal Title: Journal of Environmental Radioactivity Volume: 138 Pages: 106-115
  • DOI: 10.1016/j.jenvrad.2014.08.008
  • Peer Reviewed / Open Access
• [Journal Article] Regional quantification of muscarinic acetylcholine receptors and β-adrenoceptors in human airways (2012)
  • Author(s): Ikeda T, Anisuzzaman AS, Yoshiki H, Sasaki M, Koshiji T, Uwada J, Nishimune A, Itoh H, Muramatsu I
  • Journal Title: British Journal of Pharmacology Volume: (In press) Issue: 6 Pages: 1804-1814
  • DOI: 10.1111/j.1476-5381.2012.01881.x
  • Peer Reviewed