Study on the functions of PDZRN3, a novel regulator of adipogenesis, for the development of therapy for diabetes
| Project/Area Number |
24590323
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
General pharmacology
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| Research Institution | Yamaguchi University |
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Principal Investigator |
HONDA Takeshi 山口大学, 医学(系)研究科(研究院), 講師 (30457311)
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| Co-Investigator(Kenkyū-buntansha) |
INUI Makoto 山口大学, 大学院医学系研究科, 教授 (70223237)
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| Co-Investigator(Renkei-kenkyūsha) |
KURAMASU Atsuo 山口大学, 大学院医学系研究科, 准教授 (90302091)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | 脂肪細胞分化 / PDZRN3 / STAT5 / 3T3L1 / 前駆脂肪細胞 / STAT5b |
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| Outline of Final Research Achievements |
PDZRN3 is a novel regulator of mesenchymal progenitor cell differentiation. We previously demonstrated that PDZRN3 is essential for the differentiation of myoblasts into myotubes, whereas it suppresses the differentiation of osteoblasts. In this study, we clarified that PDZRN3 plays a pivotal role in adipocyte differentiation as a negative regulator. In 3T3-L1 cells, PDZRN3 suppressed the expression of STAT5b in a subtype-specific manner. STAT5b is a critical transcription factor for adipocyte differentiation. Furthermore, we extracted the proteins co-precipitated with PDZRN3 from the mouse embryonic tissues, and identified several candidates of a PDZRN3-interacting protein by mass spectrometry-based shotgun proteomic analysis.
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Report
(4 results)
Research Products
(7 results)
