| Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Outline of Final Research Achievements |
Type 2 ryanodine receptor is a Ca2+ release channel in the sarcoplasmic reticulum and plays an important role in excitation-contraction coupling in heart. Mutations in RyR2 cause hyperactivate the channel, leading to arrhythmogenic diseases. Here, we examined molecular mechanism of disease-cuasing mutations on the channel activity by determining the properties of the mutant channels. Furthermore, we tried to detect possible interactions within or between subdomains by introducing photo-crosslinkable artificial amino acids. We found that disease-causing mutations affect various parameters of the channel activity. Possible alterations of interactions within the domains are also suggested.
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