Elucidation of regulatory mechanisms of type 2 ryanodine receptors by introducing artificial amino acids
Project/Area Number |
24590330
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
General pharmacology
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Research Institution | Juntendo University |
Principal Investigator |
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Co-Investigator(Renkei-kenkyūsha) |
KUREBAYASHI Nagomi 順天堂大学, 医学部, 准教授 (50133335)
SAKAMOTO Kensaku 理化学研究所, 拡張遺伝暗号システム研究チーム, チームリーダー (50240685)
OBA Toshiharu 中部大学, 応用生物学部, 教授 (50008330)
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Project Period (FY) |
2012-04-01 – 2015-03-31
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Project Status |
Completed (Fiscal Year 2014)
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Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
|
Keywords | リアノジン受容体 / 筋小胞体 / カルシウムチャネル / 相互作用 / 人工アミノ酸 / 興奮収縮連関 / 不整脈 / S4-S5リンカー |
Outline of Final Research Achievements |
Type 2 ryanodine receptor is a Ca2+ release channel in the sarcoplasmic reticulum and plays an important role in excitation-contraction coupling in heart. Mutations in RyR2 cause hyperactivate the channel, leading to arrhythmogenic diseases. Here, we examined molecular mechanism of disease-cuasing mutations on the channel activity by determining the properties of the mutant channels. Furthermore, we tried to detect possible interactions within or between subdomains by introducing photo-crosslinkable artificial amino acids. We found that disease-causing mutations affect various parameters of the channel activity. Possible alterations of interactions within the domains are also suggested.
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Report
(4 results)
Research Products
(16 results)
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[Journal Article] Usefulness of running wheel for detection of congestive heart failure in dilated cardiomyopathy mouse model2014
Author(s)
Sugihara M, Odagiri F, Suzuki T, Murayama T, Nakazato Y, Unuma K, Yoshida K, Daida H, Sakurai T, Morimoto S, Kurebayashi N.
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Journal Title
PLoS One
Volume: 8(1)
Issue: 1
Pages: e55514-e55514
DOI
Related Report
Peer Reviewed
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[Journal Article] Overexpression of ryanodine receptor type 1 enhances mitochondrial fragmentation and Ca2+-induced ATP production in cardiac H9c2 myoblasts2013
Author(s)
O-Uchi J, Jhun BS, Hurst S, Bisetto S, Gross P, Chen M, Kettlewell S, Park J, Oyamada H, Smith GL, Murayama T, Sheu SS
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Journal Title
Am J Physiol Heart Circ Physiol
Volume: 305
Issue: 12
Pages: H1736-H1751
DOI
Related Report
Peer Reviewed
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[Journal Article] Mitosis-Coupled, Microtubule-Dependent Clustering of Endosomal Vesicles around Centrosomes2013
Author(s)
Takatsu, H., Katoh, Y., Ueda, T., Waguri, S., Murayama, T., Takahashi, S., Shin, H.-W. &Nakayama, K.
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Journal Title
Cell Structure and Function
Volume: 38
Issue: 1
Pages: 31-41
DOI
NAID
ISSN
0386-7196, 1347-3700
Related Report
Peer Reviewed
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[Journal Article] Sperm-associated antigen 4, a novel hypoxia-inducible factor 1 target, regulates eytokinesis, and its expression correlates with the prognosis of renal cell earcinoma.2013
Author(s)
Shoji K, Mimura I, Murayama T, Wada T, Kume H, Goto A, Ohse T, Tanaka T, Inagi R, van der Hoorn FA, Manabe I, Homma Y, Fukayama M, Sakurai T, Hasegawa T, Aburatani H, Kodama T, Nangaku M.
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Journal Title
American Journal of Pathology
Volume: 182
Issue: 6
Pages: 2191-2203
DOI
Related Report
Peer Reviewed
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[Journal Article] Multistep ion channel remodeling and lethal arrhythmia precede heart failure in a mouse model of inherited dilated cardiomyopathy2012
Author(s)
Suzuki T, Shioya T, Murayama T, Sugihara M, Odagiri F, Nakazato Y, Nishizawa H, Chugun A, Sakurai T, Daida H, Morimoto S, Kurebayashi N
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Journal Title
PLoS ONE
Volume: 7
Issue: 4
Pages: e35353-e35353
DOI
Related Report
Peer Reviewed
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