Study of the regulatory mechanism of lectin in infection and disease

• Project/Area Number: 24590345
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: General medical chemistry
• Research Institution: The Noguchi Institute (2013-2014); Sasaki Institute (2012)
• Principal Investigator: IDEO Hiroko 公益財団法人野口研究所, その他部局等, 研究員 (90180322)
• Co-Investigator(Kenkyū-buntansha): YAMASHITA Katsuko 横浜市立大学, 医学研究科, その他 (70030905)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000); Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: ガレクチン-4 / MUC-1 / 細胞外放出 / シグナル伝達分子 / 硫酸化糖鎖 / がん / レクチンELISA / ガレクチン / ＭＵＣ１ / 糖 / 感染 / 炎症 / チロシンリン酸化 / 細胞外分泌 / 感染防御 / レクチン / 感染制御 / 糖鎖認識 / 糖タンパク質輸送

Outline of Final Research Achievements

To investigate the biological function and mechanism of sugar chains in host defense system, we studied the regulatory roles of galectin-4 in infection and disease. Galectin-4 is abundantly expressed in the epithelium of the alimentary tract, however it's externalization mechanism is currently unknown. Our study showed that externalization of galectin-4 and its ligands (MUC1) can be regulated by signaling molecules, which are activated in cancer and infected cells.
Next, we developed a lectin-sandwich immunoassay using galectin-4 and a MUC1 antibody and found that galectin-4 preferred MUC1 possessing 3'-sulfated core1, which was profoundly expressed in the sera of patients with recurrent and/or metastatic breast cancer. Thus involvement of galectin-4 and its preferred sugar chain in cancer malignancy is strongly suggested.

Research Products

• [Journal Article] Expression of mucin 1 possessing a 3'-sulfated core1 in recurrent and metastatic breast cancer. (2015)
  • Author(s): Ideo H, Hinoda Y, Sakai K, Hoshi I, Yamamoto S, Oka M, Maeda K, Maeda N, Hazama S, Amano J, Yamashita K.
  • Journal Title: International Journal of Cancer Volume: 137 Issue: 7 Pages: 00-00
  • DOI: 10.1002/ijc.29520
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] High-sensitivity immunoassay with surface plasmon field-enhanced fluorescencespectroscopy using a plastic sensor chip: application to quantitative analysis of total prostate-specific antigen and GalNAcβ1-4GlcNAc-linked prostate-specific antigen for prostate cancer diagnosis. (2015)
  • Author(s): Kaya T, Kaneko T, Kojima S, Nakamura Y, Ide Y, Ishida K, Suda Y, Yamashita K.
  • Journal Title: Analitical Chemistry Volume: 87 Issue: 3 Pages: 1797-803
  • DOI: 10.1021/ac503735e
  • Peer Reviewed
• [Journal Article] Determination of glycan motifs using serial lectin affinity chromatography (2014)
  • Author(s): Yamashita K, Ohkura T.
  • Journal Title: Methods Mol Biol. Volume: 1200 Pages: 79-92
  • DOI: 10.1007/978-1-4939-1292-6_7
  • ISBN: 9781493912919, 9781493912926
  • Peer Reviewed
• [Journal Article] Intracellular Localization and Phosphorylation of Galectin-4 is Controlled by Src Family Kinases (2013)
  • Author(s): Ideo H, Hoshi I, Yamashita K, Sakamoto M.
  • Journal Title: Journal of Glycobiology Volume: 23 Issue: 12 Pages: 1452-1462
  • DOI: 10.1093/glycob/cwt073
  • Peer Reviewed
• [Presentation] DETECTION OF MUCIN 1 POSSESSING A 3’-SULFATED CORE1 IN RECURRENT AND METASTATIC BREAST CANCE (2015)
  • Author(s): Ideo H, Hinoda Y, Sakai K, Hoshi I, Yamamoto S, Oka M, Maeda K, Maeda N, Hazama S, Amano J, Yamashita K.
  • Organizer: 23rd INTERNATIONAL SYMPOSIUM ON GLYCOCONJUGATES
  • Place of Presentation: Split, Croatia
  • Year and Date: 2015-09-15 – 2015-09-20
• [Presentation] ガレクチン-4の細胞外放出にSrc family kinasesが関与する (2014)
  • Author(s): 井手尾浩子、山下克子、天野純子
  • Organizer: 日本薬学会第134回年会
  • Place of Presentation: 熊本市
• [Presentation] Intracellular Localization and Phosphorylation of Galectin-4 is Controlled by Src Family Kinases (2013)
  • Author(s): Ideo H, Hoshi I, Yamashita K, Sakamoto M.
  • Organizer: 国際複合糖質シンポジウム (GLYCO-22)
  • Place of Presentation: 大連市、中国
• [Presentation] Intracellular Localization and Phosphorylation of Galectin-4 is Controlled by Src Family Kinases (2013)
  • Author(s): 井手尾浩子
  • Organizer: 22nd International Glycoconjugate Symposium
  • Place of Presentation: 大連市、中国
• [Presentation] Functional roles of galectin-4 in malignant adenocarcinomas (2012)
  • Author(s): 井手尾浩子
  • Organizer: 第7１回日本癌学会学術総会
  • Place of Presentation: 札幌市
• [Presentation] 癌細胞の悪性度に関連したガレクチン-4及びそのリガンドの発現 (2012)
  • Author(s): 井手尾浩子
  • Organizer: 第85回日本生化学会大会
  • Place of Presentation: 福岡市
• [Book] 「糖鎖の新機能開発・応用ハンドブック｣第２章糖鎖と生命ー５免疫－５ガレクチン (2015)
  • Author(s): 山下克子、井手尾浩子 （秋吉一成監修）
  • Publisher: （株）エヌ・ティー・エス