| Project/Area Number |
24590345
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
General medical chemistry
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| Research Institution | The Noguchi Institute (2013-2014)
Sasaki Institute (2012) |
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Principal Investigator |
IDEO Hiroko 公益財団法人野口研究所, その他部局等, 研究員 (90180322)
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| Co-Investigator(Kenkyū-buntansha) |
YAMASHITA Katsuko 横浜市立大学, 医学研究科, その他 (70030905)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | ガレクチン-4 / MUC-1 / 細胞外放出 / シグナル伝達分子 / 硫酸化糖鎖 / がん / レクチンELISA / ガレクチン / ＭＵＣ１ / 糖 / 感染 / 炎症 / チロシンリン酸化 / 細胞外分泌 / 感染防御 / レクチン / 感染制御 / 糖鎖認識 / 糖タンパク質輸送 |
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| Outline of Final Research Achievements |
To investigate the biological function and mechanism of sugar chains in host defense system, we studied the regulatory roles of galectin-4 in infection and disease. Galectin-4 is abundantly expressed in the epithelium of the alimentary tract, however it's externalization mechanism is currently unknown. Our study showed that externalization of galectin-4 and its ligands (MUC1) can be regulated by signaling molecules, which are activated in cancer and infected cells.
Next, we developed a lectin-sandwich immunoassay using galectin-4 and a MUC1 antibody and found that galectin-4 preferred MUC1 possessing 3'-sulfated core1, which was profoundly expressed in the sera of patients with recurrent and/or metastatic breast cancer. Thus involvement of galectin-4 and its preferred sugar chain in cancer malignancy is strongly suggested.
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