Functional characterization of epigenetic regulatory factors identified by retroviral insertional mutagenesis

• Project/Area Number: 24590346
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: General medical chemistry
• Research Institution: Kanazawa University
• Principal Investigator: SUZUKI TAKESHI 金沢大学, がん進展制御研究所, 教授 (30262075)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000); Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: エピジェネティクス / がん遺伝子 / レトロウイルス / 分子標的 / 挿入変異 / がん分子標的 / 疾患モデルマウス

Outline of Final Research Achievements

Retroviral insertional mutagenesis in mice is one of the powerful strategies for high-throughput identification of cancer genes. Using this system, we have frequently identified the genes encoding the enzymes engaged in histone or DNA methylation. We have found that some of the enzymes are involved not only in the tumor initiation but also in the malignant tumor progression such as cell invasion and drug resistance.

Research Products

• [Journal Article] EED regulates epithelial-mesenchymal transition of cancer cells induced by TGF-beta. (2014)
  • Author(s): Oktyabri D, Tange S, Terashima M, Ishimura A and Suzuki T.
  • Journal Title: Biochem. Biophys. Res. Commun. Volume: 453 Issue: 1 Pages: 124-130
  • DOI: 10.1016/j.bbrc.2014.09.082
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] JARID2 is involved in Transforming Growth Factor-beta-induced epithelial-mesenchymal transition of lung and colon cancer cell lines. (2014)
  • Author(s): Tange S, Oktyabri D, Terashima M, Ishimura A and Suzuki T.
  • Journal Title: PLoS One Volume: 9 Issue: 12 Pages: e115684-e115684
  • DOI: 10.1371/journal.pone.0115684
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] KDM5B histone demethylase controls epithelial-mesenchymal transition of cancer cells by regulating the expression of the microRNA-200 family. (2013)
  • Author(s): Enkhbaatar Z, Yano S, et al.
  • Journal Title: Cell Cycle Volume: 12 Issue: 13 Pages: 2100-12
  • DOI: 10.4161/cc.25142
  • Peer Reviewed
• [Journal Article] Roles of histone methyl-modifying enzymes in development and progression of cancer. (2013)
  • Author(s): Suzuki T, Terashima M, Tange S, Ishimura A.
  • Journal Title: Cancer Science Volume: 104 Issue: 7 Pages: 795-800
  • DOI: 10.1111/cas.12169
  • NAID: 120005830612
  • Peer Reviewed
• [Journal Article] Jmjd5, an H3K36me2 histone demethylase, modulates embryonic cell proliferation through the regulation of Cdknla expression (2012)
  • Author(s): Ishimura, A., K-I. Minehata, M.Terashima, G.Kondoh, T.Hara, T. Suzuki
  • Journal Title: Development Volume: 139 Issue: 4 Pages: 749-759
  • DOI: 10.1242/dev.074138
  • Peer Reviewed
• [Journal Article] JMJD5, a Jumonji C(JmjC) domain-containing protein, negatively regulates osteoclastogenesis by facilitating NFATc1 protein degradation (2012)
  • Author(s): Youn, M.-Y., Imai, Y., et al.
  • Journal Title: J Biol Chem Volume: 287 Issue: 16 Pages: 12994-13004
  • DOI: 10.1074/jbc.m111.323105
  • Peer Reviewed
• [Presentation] Screening of histone modifying enzymes involved in cancer malignancies. (2014)
  • Author(s): Ishimura A, Tange S, Oktyabri D, Terashima M and Suzuki T.
  • Organizer: 第37回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2014-11-25 – 2014-11-27
• [Presentation] EED regulates epithelial-mesenchymal transition of cancer cells induced by TGF-beta. (2014)
  • Author(s): Tange S, Oktyabri D, Terashima M, Ishimura A and Suzuki T.
  • Organizer: 第37回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2014-11-25 – 2014-11-27
• [Presentation] Functional characterization of histone demethylases in the process of epithelial-mesenchymal transition of cancer cells. (2014)
  • Author(s): Suzuki T, Tange S, Oktyabri D, Terashima M and Ishimura A.
  • Organizer: 第73回日本癌学会学術総会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2014-09-25 – 2014-09-27
• [Presentation] A Role for PLU1 on epithelial-mesenchymal transition in cancer cells. (2014)
  • Author(s): Tange S, Enkhbaatar Z, Terashima M and Suzuki T.
  • Organizer: 平成26年度がん若手研究者ワークショップ
  • Place of Presentation: 蓼科グランドホテル
  • Year and Date: 2014-09-03 – 2014-09-06
• [Presentation] A role for KDM5B on epithelial-mesenchymal transition in cancer cells. (2014)
  • Author(s): Tange S, Enkhbaatar Z, Terashima M and Suzuki T.
  • Organizer: The 9th International Symposium of Institute Network.
  • Place of Presentation: 大阪大学銀杏会館
  • Year and Date: 2014-06-19 – 2014-06-20
• [Presentation] Involvement of histone demethylases in cancer identified by retroviral insertional mutagenesis. (2013)
  • Author(s): Suzuki T.
  • Organizer: SUCRI-KUCRI Joint Symposium
  • Place of Presentation: Seoul National University
  • Invited
• [Presentation] Involvement of histone methyl-modifying enzymes in cancer identified by retroviral insertional mutagenesis. (2013)
  • Author(s): Suzuki T, Tange S, Enkhbaatar Z, Oktyabri D, Terashima M and Ishimura A.
  • Organizer: 第72回日本癌学会学術総会
  • Place of Presentation: パシフィコ横浜
  • Invited
• [Presentation] Involvement of histone methyl-modifying enzymes in cancer development identified by retroviral insertional mutagenesis. (2013)
  • Author(s): Suzuki T.
  • Organizer: KUCRI-FUSCC Joint Symposium
  • Place of Presentation: Kanazawa University
  • Invited
• [Presentation] JmjCファミリー遺伝子Jmjd5による新しい遺伝子発現制御メカニズムの解析 (2013)
  • Author(s): Ishimura A, Tange S, Oktyabri D, Enkhbaatar Z, Hara T and Suzuki T.
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸国際会議場
• [Presentation] がん細胞の上皮間葉転換におけるヒストン脱メチル化酵素の機能解析 (2013)
  • Author(s): Tange S, Enkhbaatar Z, Terashima M, Oktyabri D, Ishimura A. and Suzuki T.
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸国際会議場
• [Presentation] Functional characterization of histone modifying enzymes in the process of malignant progression of cancer (2012)
  • Author(s): Suzuki T, Terashima M, Enkhbaatar Z, Oktyabri D, and Ishimura A.
  • Organizer: 第71回日本癌学会学術総会
  • Place of Presentation: 札幌市教育文化会館（北海道）
• [Presentation] Functional analysis of histone demethylases in epithelial-mesenchymal transition of cancer cells. (2012)
  • Author(s): Oktyabri D, Terashima M, Enkhbaatar Z, Tange S, Ishimura A and Suzuki T
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡国際会議場（福岡県）
• [Presentation] Jmjd5, an H3K36me2 histone demethylase, modulates embryonic cell proliferation through the regulation of p53-target genes expression. (2012)
  • Author(s): Ishimura A, Minehata K, Terashima M, Kondoh G, Hara T and Suzuki T
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡国際会議場（福岡県）
• [Book] 疾患モデルの作製と利用 がん (2012)
  • Author(s): 鈴木健之 中村卓郎編集
  • Total Pages: 633
  • Publisher: エル・アイ・シー
• [Remarks] 金沢大学がん進展制御研究所機能ゲノミクス研究分野
  • URL: http://www.kanazawa-u.ac.jp/~ganken/department/mccb/11.html