Identification of a novel steroidogenic-enzyme and analysis of its transcriptional regulation through alternations of chromatin structure
Project/Area Number |
24590347
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
General medical chemistry
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Research Institution | University of Fukui |
Principal Investigator |
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Project Period (FY) |
2012-04-01 – 2015-03-31
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Project Status |
Completed (Fiscal Year 2014)
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Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2014: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2013: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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Keywords | GSTAファミリー / SF-1 / ステロイドホルモン / クロマチン / 転写因子 / 遺伝子発現 / SF-1複合体 / C/EBPβ / プロゲステロン産生 |
Outline of Final Research Achievements |
Steroidogenic factor 1 (SF-1, also known as Ad4BP) plays a pivotal role in the regulation of expression of steroidogenesis-related genes. To identify novel SF-1-target genes, a genome-wide analysis was carried out. We found that human glutathione S-transferase A (GSTA) family genes (hGSTA1–4) are regulated by SF-1. All hGSTA genes were upregulated by transduction of SF-1 into hMSCs, while knockdown of endogenous SF-1 in H295R cells downregulated all hGSTA genes. ChIP and 3C assays revealed that the coordinated expression of the genes was based on changes in higher-order chromatin structure triggered by SF-1. In steroidogenesis, the gene products of hGSTA1 and hGSTA3 were shown to function in a coordinated fashion with 3β-HSD II. This result indicates that enzymes hGSTA1-1 and hGSTA3-3 could be considered new members of the group of SF-1 controlled steroidogenic enzymes and to participate in Δ5-Δ4 isomerization of steroid hormone precursors.
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Report
(4 results)
Research Products
(67 results)
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[Journal Article] Identification of novel steroidogenic factor 1 (SF-1)-target genes and components of the SF-1 nuclear complex2015
Author(s)
Mizutani, T., Kawabe, S., Ishikane, S., Imamichi, Y., Umezawa, A., Miyamoto, K.
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Journal Title
Molecular and Cellular Endocrinology
Volume: 408
Pages: 133-137
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] C/EBPβ (CCAAT/enhancer-binding protein β ) mediates progesterone production through transcriptional regulation in co-operation with SF-1 (steroidogenic factor-1 )2014
Author(s)
Mizutani, T., Ju, Y., Imamichi, Y., Osaki, T., Yazawa, T., Kawabe, S., Ishikane, S., Matsumura, T., Kanno, M., Kamiki, Y., Kimura, K., Minamino, N., Miyamoto, K.
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Journal Title
Biochem. J.
Volume: 460
Issue: 3
Pages: 459-471
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
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[Journal Article] Human glutathione S-transferase A (GSTA) family genes are regulated by steroidogenic factor 1 (SF-1) and are involved in steroidogenesis.2013
Author(s)
Matsumura T, Imamichi Y, Mizutani T, Ju Y, Yazawa T, Kawabe S, Kanno M, Ayabe T, Katsumata N, Fukami M, Inatani M, Akagi Y, Umezawa A, Ogata T, Miyamoto K
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Journal Title
FASEB J
Volume: 27
Issue: 8
Pages: 3198-3208
DOI
Related Report
Peer Reviewed
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[Presentation] 心房性ナトリウム利尿ペプチドのアンジオテンシンIIによるメラノーマ血行性肺転移増悪化に対する抑制作用2015
Author(s)
石兼 真, 水谷哲也, 細田洋司, 徳留 健, 野尻 崇, 三浦浩一, 木村 亨, 秋武義治, 河邉真也, 今道力敬, 宮里幹也, 寒川賢治, 宮本 薫
Organizer
日本薬学会 第135年会
Place of Presentation
兵庫医療大学(神戸市中央区)
Year and Date
2015-03-25 – 2015-03-28
Related Report
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[Presentation] 心房性ナトリウム利尿ペプチドはアンジオテンシンIIにより増悪するメラノーマ血行性肺転移を抑制する2015
Author(s)
石兼 真, 水谷哲也, 細田洋司, 徳留 健, 野尻 崇, 三浦浩一, 木村 亨, 秋武義治, 河邉真也, 今道力敬, 宮里幹也, 寒川賢治, 宮本 薫
Organizer
第88回日本薬理学会年会
Place of Presentation
名古屋国際会議場(名古屋市熱田区)
Year and Date
2015-03-18 – 2015-03-20
Related Report
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[Presentation] Atrial natriuretic peptide attenuates pulmonary metastasis enhanced by angiotensin II through regulation of tumor-cell adhesion to vascular endothelial cells2014
Author(s)
Ishikane, S., Mizutani, T., Hosoda, H., Tokudome, T., Nojiri, T., Kimura, T., Miura, K., Kawabe, S., Imamichi, Y., Miyazato, M., Kangawa, K., Miyamoto, K.
Organizer
The Conference on Bioactive Peptides for Cell-Cell Communication 2014
Place of Presentation
ホテルグランディア京都(京都市下京区)
Year and Date
2014-09-10 – 2014-09-12
Related Report
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[Presentation] Steroidogenic Factor 1 (SF-1) and C/EBPβ cooperatively regulate progesterone production2014
Author(s)
Mizutani, T., Imamichi, Y., Kawabe, S., Ishikane, S., Osaki, T., Minamino, N., Miyamoto, K.
Organizer
16th International Congress of Endocrinology. The Endocrine Society's 96th Annual Meeting & EXPO 2014
Place of Presentation
McCormic Place(米国・シカゴ)
Year and Date
2014-06-21 – 2014-06-24
Related Report
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[Presentation] ヒト生殖腺におけるHSD11B2の役割2014
Author(s)
今道力敬, 矢澤隆志, 河邉真也, 向井邦晃, 折坂 誠, 水谷哲也, 宮本 薫
Organizer
第87回日本内分泌学会学術総会
Place of Presentation
福岡国際会議場(福岡県福岡市博多区)
Year and Date
2014-04-24 – 2014-04-26
Related Report
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