| Project/Area Number |
24590350
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
General medical chemistry
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| Research Institution | Toyohashi University of Technology |
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Principal Investigator |
NUMANO Rika 豊橋技術科学大学, 工学(系)研究科(研究院), 准教授 (30462716)
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| Co-Investigator(Kenkyū-buntansha) |
NAKAO Kazuki 東京大学, 医学(系)研究科(研究院), 准教授 (20217657)
KAWANO Takesi 豊橋技術科学大学, 工学(系)研究科(研究院), 准教授 (70452216)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2014: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | 概日リズム / 電極プローブ / 光刺激 / 極小電極VLSチップ / 視交叉上核 / 時計遺伝子Per1 / Per1組み換えマウス / 豊橋プローブ / 深部細胞 / SCNslice / VLSプローブチップ |
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| Outline of Final Research Achievements |
All living things use internal clocks with the24 hr period, circadian rhythms.Period1 (Per1) gene is an important member of the central clock system in the SCN. I observed Per1::GFP transgenic mice SCN to monitor circadian rhythms by Per1 rhythmic expression.
Dynamic simulation system for functional map in the SCN can be established to activate by pinpoint stimulation by 1)TOYOHASHI Vapor-liquid-solid micro electrical array chip has nano-scale electrical probes with 1micro m diameter and can be used for both detecting the electric activity and stimulating neural activity in one cell level. TOYOHASHI probes could insert into deeply brain.2) LiGluR for rapid, remote and reversible control of cell membrane potential by photo illumination and photoiosomerizable chemicals.The fibroblast cell lines transfected by Per1:GFP and by LiGluR showed to induce Per1 transcription after UV illumination. The signal were transfered in the SCN slice for several hours.
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