Identification of conversion of cells by proteomics analysis
Project/Area Number |
24590353
|
Research Category |
Grant-in-Aid for Scientific Research (C)
|
Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
General medical chemistry
|
Research Institution | Kyoto University |
Principal Investigator |
|
Project Period (FY) |
2012-04-01 – 2015-03-31
|
Project Status |
Completed (Fiscal Year 2014)
|
Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
|
Keywords | 質量分析 / 幹細胞 / iPS細胞 / 定量 / 初期化 / ES細胞 / プロテオミクス / プロテオーム解析 / 転写因子 / 分画 |
Outline of Final Research Achievements |
We have been studying for the purpose of capturing the expression changes of proteins during the conversion process of the cell. We proceeded improvements in identification techniques for proteins using a mass spectrometer (MS). First, using a meter-long monolithic column in order to improve the separation of the sample. Second, cells were fractionated into the cell membrane, cytoplasm, nuclear. We were able to achieve an increase in the number of protein that can be finally identified. Furthermore, by combining with labeling for quantitative comparing between samples, it was possible to carry out the establishment of accurately quantifying technology less samples with high sensitivity. Was subjected to analysis of human iPS cells and the original fibroblasts using this technique and have enabled the identification of gene which identification was difficult until now.
|
Report
(4 results)
Research Products
(16 results)
-
-
[Journal Article] Derivation of mesenchymal stromal cells from pluripotent stem cells through a neural crest lineage using small molecule compounds with defined media.2014
Author(s)
Fukuta M, Nakai Y, Kirino K, Nakagawa M, Sekiguchi K, Nagata S, Matsumoto Y, Yamamoto T, Umeda K, Heike T, Okumura N, Koizumi N, Sato T, Nakahata T, Saito M, Otsuka T, Kinoshita S, Ueno M, Ikeya M, Toguchida J.
-
Journal Title
PLOS ONE
Volume: 9
Issue: 12
Pages: e112291-e112291
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
-
[Journal Article] A novel efficient feeder-free culture system for the derivation of human induced pluripotent stem cells.2014
Author(s)
Nakagawa M, Taniguchi Y, Senda S, Takizawa N, Ichisaka T, Asano K, Morizane A, Doi D, Takahashi J, Nishizawa M, Yoshida Y, Toyoda T, Osafune K, Sekiguchi K, YamanakaS.
-
Journal Title
Scientific Reports
Volume: 4
Issue: 1
Pages: 3594-3594
DOI
NAID
Related Report
Peer Reviewed
-
-
[Journal Article] A chemical probe that labels human pluripotent stem cells2014
Author(s)
Hirata N, Nakagawa M, Fujibayashi Y, Yamauchi K, Murata A, Minami I, Tomioka M, Kondo T, Kuo TF, Endo H, Inoue H, Sato S, Ando S, Kawazoe Y, Aiba K, Nagata K, Kawase E, Chang YT, Suemori H, Eto K, Nakauchi H, Yamanaka S, Nakatsuji N, Ueda K, Uesugi M
-
Journal Title
Cell reports
Volume: 6
Issue: 6
Pages: 1165-74
DOI
NAID
Related Report
Peer Reviewed / Open Access
-
-
-
-
-
-
-
-
-
-
-