Functional analysis of PLA/AT family members functioning as lipid-metabolizing enzymes
| Project/Area Number |
24590355
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
General medical chemistry
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| Research Institution | Kagawa University |
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Principal Investigator |
UYAMA Toru 香川大学, 医学部, 助教 (30457337)
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| Co-Investigator(Renkei-kenkyūsha) |
UEDA Natsuo 香川大学, 医学部, 教授 (20193807)
TSUBOI Kazuhito 香川大学, 医学部, 助教 (80346642)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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| Keywords | PLA/ATファミリー / HRASLSファミリー / 脂質代謝酵素 / アシル転移酵素 / ホスホリパーゼA1/A2 / リン脂質 / N-アシルホスファチジルエタノールアミン / ペルオキシソーム / N-アシルエタノールアミン |
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| Outline of Final Research Achievements |
We investigated possible lipid-metabolizing activities of PLA/AT (HRASLS) family members which were originally identified as tumor suppressor genes. We found that the expression of PLA/AT-1, -2, -4 and -5 causes the formation of N-acylphosphatidylethanolamine in living cells. To further examine their roles, HEK293 cells expressing PLA/AT-1 or PLA/AT-2 stably were established. Liquid chromatography-tandem mass spectrometry confirmed significant increases in N-acylphosphatidylethanolamine levels in these cells. These results suggest that PLA/AT-1 and -2 are enzymes responsible for the generation of N-acylphosphatidylethanolamine in mammalian cells.
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Report
(4 results)
Research Products
(26 results)
