| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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| Outline of Final Research Achievements |
We investigated possible lipid-metabolizing activities of PLA/AT (HRASLS) family members which were originally identified as tumor suppressor genes. We found that the expression of PLA/AT-1, -2, -4 and -5 causes the formation of N-acylphosphatidylethanolamine in living cells. To further examine their roles, HEK293 cells expressing PLA/AT-1 or PLA/AT-2 stably were established. Liquid chromatography-tandem mass spectrometry confirmed significant increases in N-acylphosphatidylethanolamine levels in these cells. These results suggest that PLA/AT-1 and -2 are enzymes responsible for the generation of N-acylphosphatidylethanolamine in mammalian cells.
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