| Project/Area Number |
24590363
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
General medical chemistry
|
|---|
| Research Institution | Fujita Health University |
|---|
Principal Investigator |
|
|---|
| Research Collaborator |
NAKATANI Masashi 藤田保健衛生大学, 総合医科学研究所, 助教 (00421264)
HITACHI Keisuke 藤田保健衛生大学, 総合医科学研究所, 助教 (10508469)
UEZUMI Akiyoshi 藤田保健衛生大学, 総合医科学研究所, 講師 (60434594)
|
|---|
| Project Period (FY) |
2012-04-01 – 2015-03-31
|
| Project Status |
Completed (Fiscal Year 2014)
|
| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
|
|---|
| Keywords | 筋分化 / 筋衛星細胞 / 間葉系前駆細胞 / 脂肪分化 / 筋萎縮 / miRNA / lncRNA / 筋疾患 / 間葉系幹細胞 / 脂肪化 / 幹細胞システム / 細胞分化 / 小分子RNA / 幹細胞 / 筋サテライト細胞 / 再生医療 / 異所性骨化 / 線維化 |
|---|
| Outline of Final Research Achievements |
Muscle atrophies are observed both in genetic and non-genetic muscular disorders. Satellite cells play an important role in muscle repair, regeneration and muscle mass. Recently, non-myogenic mesenchymal progenitors/stem cells have been identified in muscles. They do not adopt a myogenic fate, however, they differentiate to adipocytes, fibroblasts and osteogenic cells. We have established an elegant method to purify myogenic cells and mesenchymal stem cells. Myostatin, a muscle-specific TGF-beta superfamily, is a pivotal negative regulator of muscle mass. Using myostatin knockout mice, we investigated the global microRNA expression profile. miR486, a positive regulator of IGF-1/Akt pathway, was, identified as a target of myostatin signaling. Overexpression of miR486 induced myotube hypertrophy. miR486 is one of the intermediate molecules linking myostatin signaling and the IGF-1/Akt/mTOR pathway in the regulation of skeletal muscle size.
|
