Regulation of pluripotency of skeletal muscle-derived stem cells and application to muscular diseases
| Project/Area Number |
24590363
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
General medical chemistry
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| Research Institution | Fujita Health University |
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Principal Investigator |
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| Research Collaborator |
NAKATANI Masashi 藤田保健衛生大学, 総合医科学研究所, 助教 (00421264)
HITACHI Keisuke 藤田保健衛生大学, 総合医科学研究所, 助教 (10508469)
UEZUMI Akiyoshi 藤田保健衛生大学, 総合医科学研究所, 講師 (60434594)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | 筋分化 / 筋衛星細胞 / 間葉系前駆細胞 / 脂肪分化 / 筋萎縮 / miRNA / lncRNA / 筋疾患 / 間葉系幹細胞 / 脂肪化 / 幹細胞システム / 細胞分化 / 小分子RNA / 幹細胞 / 筋サテライト細胞 / 再生医療 / 異所性骨化 / 線維化 |
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| Outline of Final Research Achievements |
Muscle atrophies are observed both in genetic and non-genetic muscular disorders. Satellite cells play an important role in muscle repair, regeneration and muscle mass. Recently, non-myogenic mesenchymal progenitors/stem cells have been identified in muscles. They do not adopt a myogenic fate, however, they differentiate to adipocytes, fibroblasts and osteogenic cells. We have established an elegant method to purify myogenic cells and mesenchymal stem cells. Myostatin, a muscle-specific TGF-beta superfamily, is a pivotal negative regulator of muscle mass. Using myostatin knockout mice, we investigated the global microRNA expression profile. miR486, a positive regulator of IGF-1/Akt pathway, was, identified as a target of myostatin signaling. Overexpression of miR486 induced myotube hypertrophy. miR486 is one of the intermediate molecules linking myostatin signaling and the IGF-1/Akt/mTOR pathway in the regulation of skeletal muscle size.
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Report
(4 results)
Research Products
(56 results)
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[Journal Article] Identification and characterization of PDGFRα + mesenchymal progenitors in human skeletal muscle.2014
Author(s)
Uezumi A, Fukada S, Yamamoto N, Ikemoto-Uezumi M, Nakatani M, Morita M, Yamaguchi A, Yamada H, Nishino I, Y Hamada Y, Tsuchida K.
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Journal Title
Cell Death Disease
Volume: 5
Issue: 4
Pages: e1184-e1184
DOI
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Peer Reviewed
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[Journal Article] An inhibitor of transforming growth factor beta type I receptor ameliorates muscle atrophy in a mouse model of caveolin 3-deficient muscular dystrophy2012
Author(s)
Ohsawa Y, Okada T, Nishimatsu SI, Ishizaki M, Suga T, Fujino M, Murakami T, Uchino M, Tsuchida K, Noji S, Hinohara A, Shimizu T, Shimizu K, Sunada Y
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Journal Title
Lab Invest
Volume: 78
Issue: 8
Pages: 1100-1114
DOI
Related Report
Peer Reviewed
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[Presentation] ヒト筋幹細胞移植を目指した基礎的研究2015
Author(s)
中村美紀, 村上聡, 上住聡芳, 佐藤貴彦, 土田邦博, 辻川和丈, 深田宗一朗
Organizer
第１４回日本再生医療学会総会
Place of Presentation
パシフィコ横浜
Year and Date
2015-03-19 – 2015-03-21
Related Report
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[Presentation] 骨格筋分泌因子の探索2014
Author(s)
中谷直史, 上田洋司, 土田邦博
Organizer
第３５回日本肥満学会
Place of Presentation
宮崎
Year and Date
2014-10-24 – 2014-10-25
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