Roles of cell adhesion molecules in enhanced cell motility induced by hypoxia-inducible factor HIF

• Project/Area Number: 24590364
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: General medical chemistry
• Research Institution: Aichi Medical University
• Principal Investigator: KANNAGI Reiji 愛知医科大学, 公私立大学の部局等, 客員教授 (80161389)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 生体分子医学 / 低酸素誘導因子 / 低酸素 / CD44 / ヒアルロン酸 / 細胞運動能 / Boydenチェンバーアッセイ / 創傷治癒アッセイ / アイソザイム / 蛍光基質 / 転写調節 / HIF-1α / ChIPアッセイ / レポーターアッセイ / セレクチン / DNAマイクロアレイ / shRNA / ホーミング

Outline of Final Research Achievements

Cellular hypoxia remarkably enhances cellular mobility. In the previous project we had found alterations in the expression of various cell adhesion molecules. In the current project we intended to identify which adhesion molecule among them is mainly responsible for the enhancement of cellular mobility induced by hypoxia. Our results indicated that the changes occurred in the CD44-hyaluronan cell adhesion system, especially altered transcription levels of the genes involved in the synthesis and catabolism of hyaluronan induced by hypoxia-inducible factor (HIF), are mainly responsible for the enhanced cellular motility under hypoxia.

Research Products

• [Journal Article] Therapeutic adenoviral gene transfer of a glycosyltransferase for prevention of peritoneal dissemination and metastasis of gastric cancer (2014)
  • Author(s): Kawamura, Y. I., et al.
  • Journal Title: Cancer Gene Therapy Volume: 21 Issue: 10 Pages: 427-433
  • DOI: 10.1038/cgt.2014.46
  • Peer Reviewed / Open Access
• [Journal Article] Introduction to special Issue: Emerging Roles of Siglecs in Health and Disease (2014)
  • Author(s): Crocker, P. R. et al.
  • Journal Title: Glycobiology Volume: 24 Issue: 9 Pages: 784-784
  • DOI: 10.1093/glycob/cwu073
  • Peer Reviewed
• [Journal Article] Individual profiles of free ceramide species and the constituent ceramide species of sphingomyelin and neutral glycosphingolipid and their alteration according to the sequential changes of environmental oxygen content in human colorectal cancer Caco-2 cells (2014)
  • Author(s): Tanaka, K., et al.
  • Journal Title: Glycoconj. J. Volume: 31 Issue: 3 Pages: 209-219
  • DOI: 10.1007/s10719-013-9511-9
  • Peer Reviewed
• [Journal Article] KSGal6ST generates galactose-6-O-sulfate in high endothelial venules but does not contribute to L-selectin-dependent lymphocyte homing (2013)
  • Author(s): Patnode, M. L., et al.
  • Journal Title: Glycobiology Volume: 23 Issue: 3 Pages: 381-394
  • DOI: 10.1093/glycob/cws166
  • Peer Reviewed
• [Journal Article] Sialic acid cyclization of human Th homing receptor glycan associated with recurrent exacerbations of atopic dermatitis (2012)
  • Author(s): Sakuma, K., et al.
  • Journal Title: J Dermatol Sci. Volume: 68 Issue: 3 Pages: 187-193
  • DOI: 10.1016/j.jdermsci.2012.09.015
  • Peer Reviewed
• [Journal Article] Transcription factors c-MYC and CDX2 mediate E-selectin ligand expression in colon cancer cells undergoing EGF/bFGF-induced epithelial-mesenchymal transition (2012)
  • Author(s): Sakuma K, Aoki M, Kannagi R.
  • Journal Title: Proc Natl Acad Sci USA Volume: 109(20) Issue: 20 Pages: 7776-7781
  • DOI: 10.1073/pnas.1111135109
  • Peer Reviewed
• [Journal Article] Induction of 6-sulfated glycans with cell adhesion activity via T-bet and GATA-3 in human helper T cells (2012)
  • Author(s): Sakuma K, Chen GY, Aoki M, Kannagi R.
  • Journal Title: Biochim Biophys Acta Volume: 1820 Issue: 7 Pages: 841-848
  • DOI: 10.1016/j.bbagen.2012.03.005
  • Peer Reviewed
• [Journal Article] Role of down-regulated neutral ceramidase during all-trans retinoic acid induced neuronal differentiation in SH-SY5Y neuroblastoma cells (2012)
  • Author(s): Kouji Tanaka
  • Journal Title: J.Biochem. Volume: (印刷中) Issue: 6 Pages: 611-620
  • DOI: 10.1093/jb/mvs033
  • Peer Reviewed
• [Presentation] T-およびB-リンパ球のホーミング糖鎖の活性調節機構と病態的意義 (2014)
  • Author(s): 神奈木玲児
  • Organizer: 糖鎖免疫 Glycoimmunology 2014 (東京医科歯科大学難治疾患共同研究拠点研究集会)
  • Place of Presentation: 東京
  • Invited
• [Presentation] Sialic acid cyclization of human Th2 homing receptor glycan associated with recurrent exacerbations of atopic dermatitis (2012)
  • Author(s): Sakuma K., et al.
  • Organizer: The 9th international conference on sialic acid, SialoGlyco 2012
  • Place of Presentation: Taipei, Taiwan
• [Book] Glycoscience: Biology and Medicine (2015)
  • Author(s): Kannagi, R., et al.
  • Publisher: Springer, Tokyo
• [Book] Fucosyltransferase 5. GDP-Fucose Lactosamine α3/4-Fucosyltransferase (FUT5). In Handbook of Glycosyltransferases and Related Genes (2014)
  • Author(s): Kannagi, R.
  • Publisher: Springer Verlag
• [Book] Fucosyltransferase 6. GDP-Fucose Lactosamine α3-Fucosyltransferase (FUT6). In Handbook of Glycosyltransferases and Related Genes (2014)
  • Author(s): Kannagi, R.
  • Publisher: Springer Verlag
• [Book] Cell-surface glycoconjugates controlling human T-lymphocyte homing: Implications for bronchial asthma and atopic dermatitis. In Chembiomolecular Science: At the Frontier of Chemistry and Biology (2013)
  • Author(s): Kannagi, R.
  • Publisher: Springer Verlag