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Analyses of molecular mechanism for regulating gene expression of anti-inflammatory NLR family member PYNOD

Research Project

Project/Area Number 24590374
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Pathological medical chemistry
Research InstitutionKanazawa University

Principal Investigator

IMAMURA Ryu  金沢大学, がん進展制御研究所, 助教 (10311680)

Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
KeywordsNLRファミリー / 自然免疫 / PYNOD / 胃がん / 遺伝子発現 / NLRファミリー蛋白
Outline of Final Research Achievements

Many members of the nucleotide-binding and oligomerization domain (NOD) and leucine-rich-repeat (LRR)-containing protein (NLR) family play important roles in pathogen recognition and inflammation. We identified PYNOD/NLRP10, one of the member of this family that lacks LRR, and found that PYNOD inhibit inflammatory signal mediated by caspase-1 and ASC. To further investigate physiological function of PYNOD, we have established PYNOD-deficient mice. PYNOD-deficient mice exhibited no obvious gross abnormalities, no evidence of autoimmunity and spontaneous tumor formation, and normal innate immune responses.
However, we have found that PYNOD is highly expressed in stomach of gastric cancer mouse model (Gan mice), in which inflammatory COX-2/PGE2 pathway and Wnt signaling are activated simultaneously in gastric mucosa. Furthermore, we also detected high PYNOD expression in tumor region containing papillary type adenocarcinoma from gastric cancer patients.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • 2012 Research-status Report
  • Research Products

    (20 results)

All 2015 2014 2013 2012 Other

All Journal Article (4 results) (of which Peer Reviewed: 4 results,  Open Access: 1 results,  Acknowledgement Compliant: 1 results) Presentation (9 results) Book (1 results) Remarks (6 results)

  • [Journal Article] NLRP3 Mediates NF-κB Activation and Cytokine Induction in Microbially Induced and Sterile Inflammation.2015

    • Author(s)
      Kinoshita, T., Imamura, R., Kushiyama, H., and Suda, T.
    • Journal Title

      PLoS One

      Volume: in press Issue: 3 Pages: 1-15

    • DOI

      10.1371/journal.pone.0119179

    • NAID

      120005611526

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Pyroptotic cells externalize eat-me and release find-me signals and are efficiently engulfed by macrophages.2013

    • Author(s)
      Wang, Q., Imamura, R., Motani, K., Kushiyama, H., Nagata, S. and Suda, T.
    • Journal Title

      Int Immunol.

      Volume: 25 Issue: 6 Pages: 363-372

    • DOI

      10.1093/intimm/dxs161

    • Related Report
      2013 Research-status Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Fas Ligand Has a Greater Impact than TNF-α on Apoptosis and Inflammation in Ischemic Acute Kidney Injury2012

    • Author(s)
      Furuichi K, Kokubo S, Hara A, Imamura R, Wang Q, Kitajima S, Toyama T, Okumura T, Matsushima K, Suda T, Mukaida N, Kaneko S, Wada T
    • Journal Title

      Nephron Extra

      Volume: 2 Issue: 1 Pages: 27-38

    • DOI

      10.1159/000335533

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Roles of the PI3 K/Akt pathway and autophagy in TLR3 sig naling-induced apoptosis and growth arr est of human prostate cancer cells2012

    • Author(s)
      Harashima, N., Inao, T., Imamura, R., et. al.
    • Journal Title

      Cancer Immunol. Immunother

      Volume: (in press) Issue: 5 Pages: 667-676

    • DOI

      10.1007/s00262-011-1132-1

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Presentation] HDAC inhibitors (HDACI) induce IL-1βproduction in human monocytes by activating NLRP3 inflammasome.2015

    • Author(s)
      Kushiyama, H., Imamura, R., Kinoshita, T., and Suda, T.
    • Organizer
      日本癌学会シンポジウム/共同利用・共同研究拠点シンポジウム
    • Place of Presentation
      石川県立音楽堂 (石川)
    • Year and Date
      2015-01-21
    • Related Report
      2014 Annual Research Report
  • [Presentation] Migration of skin antigen-transporting cells in PYNOD-deficient mice.2014

    • Author(s)
      Yoshino, M., Imamura, R., Murata, A., Shimoda, Y., Hikosaka, M., Suda, T., and Hayashi, S.
    • Organizer
      第43回日本免疫学会総会・学術集会
    • Place of Presentation
      国立京都国際会館 (京都)
    • Year and Date
      2014-12-10
    • Related Report
      2014 Annual Research Report
  • [Presentation] Role of PYNOD (NLRP10) in the migration of effector T cells to inflamed tissues.2014

    • Author(s)
      Imamura, R., and Suda, T.
    • Organizer
      第43回日本免疫学会総会・学術集会
    • Place of Presentation
      国立京都国際会館 (京都)
    • Year and Date
      2014-12-10
    • Related Report
      2014 Annual Research Report
  • [Presentation] がん細胞における自然免疫センサー分子NLRP3の役割2014

    • Author(s)
      木下 健、今村 龍、須田貴司
    • Organizer
      第37回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜 (神奈川)
    • Year and Date
      2014-11-25
    • Related Report
      2014 Annual Research Report
  • [Presentation] HDAC阻害剤はヒト単球にNLRP3インフラマソーム依存性のIL-1β産生を誘導する2014

    • Author(s)
      Kushiyama, H., Suda, T., Imamura, R., and Kinoshita, T.
    • Organizer
      第73回日本癌学会学術総会
    • Place of Presentation
      パシフィコ横浜 (神奈川)
    • Year and Date
      2014-09-27
    • Related Report
      2014 Annual Research Report
  • [Presentation] Trichostatin A induces macrophage IL-1β production by activating NLRP3 inflammasome.2014

    • Author(s)
      Kusiyama, H., Imamura, R., Kinoshita, T., and Suda, T.
    • Organizer
      International Symposium on Tumor Biology in Kanazawa & Symposium on Drug Discovery in Academics
    • Place of Presentation
      Excel Hotel Tokyu (金沢)
    • Related Report
      2013 Research-status Report
  • [Presentation] Functional analyses of PYNOD (NLRP10) in mice2013

    • Author(s)
      Imamura, R. and Suda, T.
    • Organizer
      3rd International Symposium on Carcinogenic Spiral and International Symposium on Tumor Biology in Kanazawa
    • Place of Presentation
      Kanazawa Excel Hotel Tokyu
    • Related Report
      2012 Research-status Report
  • [Presentation] Functional analyses of PYNOD (NLRP10) in mice2012

    • Author(s)
      Imamura, R. and Suda, T.
    • Organizer
      第41回日本免疫学会学術集会
    • Place of Presentation
      神戸国際会議場
    • Related Report
      2012 Research-status Report
  • [Presentation] The role of NLRP3 in the induction of cytokine gene expression in tumor cells2012

    • Author(s)
      Kinoshita, T., Imamura, R., and Suda, T,
    • Organizer
      第35回日本分子生物学会年会
    • Place of Presentation
      福岡国際会議場
    • Related Report
      2012 Research-status Report
  • [Book] サイトカインのすべて インターロイキン14 (IL-14)2012

    • Author(s)
      今村 龍
    • Total Pages
      6
    • Publisher
      科学評論社
    • Related Report
      2012 Research-status Report
  • [Remarks] がん進展制御研究所ホームページ

    • URL

      http://www.kanazawa-u.ac.jp/~ganken

    • Related Report
      2014 Annual Research Report
  • [Remarks] がん進展制御研究所・腫瘍動態制御研究部門ホームページ

    • URL

      http://www.kanazawa-u.ac.jp/~ganken/shuyoudoutaiseigyo/index.html

    • Related Report
      2014 Annual Research Report
  • [Remarks] がん進展制御研究所ホームペーシ

    • URL

      http://www.kanazawa-u.ac.jp/̃ganken

    • Related Report
      2013 Research-status Report
  • [Remarks] 免疫炎症制御研究分野ホームペーシ

    • URL

      http://web.kanazawa-u.ac.jp/̃cdmtd/index.html

    • Related Report
      2013 Research-status Report
  • [Remarks] がん進展制御研究所ホームページ

    • URL

      http://www.kanazawa-u.ac.jp/~ganken

    • Related Report
      2012 Research-status Report
  • [Remarks] 免疫炎症制御研究分野ホームページ

    • URL

      http://web.kanazawa-u.ac.jp/~cdmtd/index.html

    • Related Report
      2012 Research-status Report

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Published: 2013-05-31   Modified: 2019-07-29  

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