Mechanism of hypoxia-induced transformation in endothelial cells
Project/Area Number |
24590381
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Pathological medical chemistry
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Research Institution | Tottori University |
Principal Investigator |
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Co-Investigator(Kenkyū-buntansha) |
TAMAKI Toshiaki 徳島大学, 大学院ヘルスバイオサイエンス研究部, 教授 (80179879)
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Project Period (FY) |
2012-04-01 – 2015-03-31
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Project Status |
Completed (Fiscal Year 2014)
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Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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Keywords | 血管内皮細胞 / 低酸素応答 / HIF / 肺高血圧症 / 転写因子 / 微小環境 / 酸化ストレス / 活性酸素 / 血管リモデリング |
Outline of Final Research Achievements |
Hypoxia-inducible factors (HIFs) in endothelial cells (ECs) help to maintain ECs and promote vascularization, and HIF-2α is abun- dantly expressed in ECs. However, the mechanisms of action of HIF-2α in ECs are not yet fully understood. The aim of this study was to evaluate the in vivo effects of overexpression of HIF-2α in ECs on skin flap survival. A random pattern skin flap was elevated on the dorsum of transgenic mice (Tg mice) with EC-specific HIF-2α conditional overexpression and wild-type littermate control mice. The HIF-2α mRNA and protein levels were significantly increased in the Tg mice when compared with control mice. Tg mice had significantly increased skin flap survival areas when compared with wild- type mice. Moreover, histological examination revealed an increase in the subcutaneous blood vessel counts in the Tg mice. Specific overexpression of HIF-2α in ECs promoted vascu- larization and enhanced skin flap survival in vivo in a mouse model.
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Report
(4 results)
Research Products
(30 results)
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[Journal Article] Overexpressed HIF-2α in Endothelial Cells Promotes Vascularization and Improves Random Pattern Skin Flap Survival.2014
Author(s)
Morimoto A, Tomita S, Imanishi M, Shioi G, Kihira Y, Izawa-Ishizawa Y, Takaku M, Hashimoto I, Ikeda Y, Nakanishi H, Tamaki T.
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Journal Title
Plast Reconstr Surg Glob Open
Volume: 7
Issue: 4
Pages: e132-e132
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Deletion of Hypoxia-Inducible Factor-1α in Adipocytes Enhances Glucagon-Like Peptide-1 Secretion and Reduces Adipose Tissue Inflammation2014
Author(s)
Yoshitaka KIHIRA, Mariko MIYAKE, Manami HIRATA, Yoji HOSHINA, Kana KATO, Hitoshi SHIRAKAWA, Hiroshi SAKAUE, Noriko YAMANO, Yuki IZAWA-ISHIZAWA, Keisuke ISHIZAWA, Yasumasa IKEDA, Koichiro TSUCHIYA, Toshiaki TAMAKI, Shuhei TOMITA
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Journal Title
PLOS ONE
Volume: 9
Issue: 4
Pages: e93856-e93856
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Olmesartan ameliorates urinary dysfUnction in the spontaneously hypertensive rat via recovering bladder blood flow and decreasing oxidative stress2014
Author(s)
Shimizu S, Saito M, Oiwa H, Ohmasa F, Tsounapi P, Oikawa R, Dimitriadis F, Martin TD, Satoh I, Kinoshita Y, Tomita S
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Journal Title
Neurourology and Urodynamics
Volume: 33
Issue: 3
Pages: 350-7
DOI
Related Report
Peer Reviewed
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[Journal Article] Role of macrophage-derived hypoxia-inducible factor (HIF)-1α as a mediator of vascular remodelling2013
Author(s)
Nakayama T, Kurobe H, Sugasawa N, Kinoshita H, Higashida M, Matsuoka Y, Yoshida Y, Hirata Y, Sakata M, Maxfield M, Shimabukuro M, Takahama Y, Sata M, Tamaki T, Kitagawa T, Tomita S.
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Journal Title
Cardiovasc Res
Volume: 99
Issue: 4
Pages: 705-715
DOI
Related Report
Peer Reviewed
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