| Project/Area Number |
24590401
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Human genetics
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| Research Institution | Kobe University |
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Principal Investigator |
HOSHI NOBUHIKO 神戸大学, (連合)農学研究科(研究院), 教授 (10209223)
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| Co-Investigator(Kenkyū-buntansha) |
YOKOYAMA Toshifumi 神戸大学, 大学院農学研究科, 助教 (10380156)
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| Co-Investigator(Renkei-kenkyūsha) |
TABUCHI Yoshiaki 富山大学, 生命科学先端研究センター (20322109)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | 性分化 / 性差 / 性特異的遺伝子発現 / SRY / SOX9 / エピジェネティック制御 / sex reversal / poschiavinus / C57BL/6 / Sox9 / Sry / 性的可塑性 / 性決定 / ニワトリ / 雄化遺伝子 / 性差構築 / 雌化遺伝子 / 分子形態学 / 性的二型性 / Ypos / 性分化関連遺伝子 / 精巣化 / 卵巣化 / 性逆転 |
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| Outline of Final Research Achievements |
Mammalian sexual fate is determined by the presence or absence of Sry in the bipotential gonads. Breeding the Y chromosome from Mus m. domesticus poschiavinus (pos) strains into C57BL/6J (B6J) mice (B6J-XYpos) has been shown to induce sex reversal. However, our C57BL/6N (B6N)-XYpos mice develop as males as well as females and true hermaphrodites. The onsets of both Sry and Sox9 expressions as determined spatiotemporally by whole-mount immunohistochemistry in the B6N-XYpos gonads occurred 2-3 tail somites later than those in B6N-XYB6 gonads, but earlier than those in B6J-XYpos, respectively. Our study is the first to histologically show the expression and ectopic localization of a female-related gene in the XYpos testes, and a male-related gene in the XYpos ovaries. The results from these and previous experiments indicate that the interplay between genome variants, epigenetics, and developmental gene regulation is crucial for testis development.
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