Early pathological evaluation for irradiation effectiveness in uterine cervical cancer
Project/Area Number |
24590406
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Human pathology
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Research Institution | Hokkaido University |
Principal Investigator |
TANINO Mishie 北海道大学, 医学(系)研究科(研究院), 講師 (90360908)
|
Co-Investigator(Kenkyū-buntansha) |
HIDEMICHI Watari 北海道大学, 北海道大学病院, 講師 (10344508)
SHINYA Tanaka 北海道大学, 大学院医学研究科, 教授 (70261287)
RUMIKO Kinoshita 北海道大学, 北海道大学病院, 助教 (70507582)
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Project Period (FY) |
2012-04-01 – 2015-03-31
|
Project Status |
Completed (Fiscal Year 2014)
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Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
|
Keywords | 子宮頸癌 / 放射線治療 / 上皮間葉移行 / 放射線照射 / 耐性 / Hippo pathway / 放射線 |
Outline of Final Research Achievements |
Ionizing irradiation is an effective treatment for uterine cervical cancer, however, a higher rate of recurrence with more aggressive phenotypes is a vital issue. Although epithelial-mesenchymal transition (EMT) is involved in irradiation-induced cancer progression, the role for such phenotypic transition in uterine cervical cancer remains unknown. To investigate the mechanism of irradiation-dependent tumor progression, cervical cancer cell lines were irradiated multifractionated 20Gy in two weeks. Knocking down Snail and Yap cell lines were established. EMT regulators, Hippo pathway effector, mesenchymal and stemness proteins were increased after irradiation. In phenotypical analysis, cells exhibited an upregulation of migration, invasion, numbers of focal adhesion. Knocking down Snail and Yap inhibit increase in mesenchymal markers. In conclusion, we propose inhibiting both EMT and Hippo pathway is useful to prevent malignat transtion after irradiation.
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Report
(4 results)
Research Products
(58 results)
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[Journal Article] Rapid immunohistochemistry based on alternating current electric field for intraoperative diagnosis of brain tumors.2015
Author(s)
Tanino M, Sasajima T, Nanjo H, Akesaka S, Kagaya M, Kimura T, Ishida Y, Oda M, Takahashi M, Sugawara T, Yoshioka T, Nishihara H, Akagami Y, Goto A, Minamiya Y, Tanaka S
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Journal Title
Brain Tumor Pathol
Volume: 32
Pages: 12-19
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
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[Journal Article] Sustained elevation of Snail promotes Glial-Mesenchymal Transition (GMT) after irradiation in malignant glioma.2014
Author(s)
Mahabir R, Tanino M, Elimansuri, Wang L, Kimura T, Itoh T, Ohba Y, Nishihara H, Shirato H, Tsuda M, Tanaka S
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Journal Title
Neuro-Oncology
Volume: 16
Pages: 671-685
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
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[Journal Article] Immunohistochemical evaluation of O6-methylguanine DNA methyltransferase (MGMT) expression in 117 cases of glioblastoma.2014
Author(s)
Miyazaki M, Nishihara H, Terasaka S, Kobayashi H, Yamaguchi S, Ito T, Kamoshima Y, Fujimoto S, Kaneko S, Katoh M, Ishii N, Mohri H, Kimura T, Tanino M, Tanaka S
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Journal Title
Neuropathology
Volume: 7
Pages: 268-276
DOI
Related Report
Peer Reviewed
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[Journal Article] 31.Epiregulin enhances tumorigenicity activating ERK/MAPK pathway in glioblastoma.2014
Author(s)
Kohsaka S, Hinohara K, Wang L, Nishimura T, Urushido M, Yachi K, Tsuda M, Tanino M, Kimura T, Nishihara H, Gotoh N, and Tanaka S
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Journal Title
Neuro-Oncology
Volume: 16
Pages: 960-970
Related Report
Peer Reviewed
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[Journal Article] (18)F-FDG PET/CT imaging for a gastrointestinal mantle cell lymphoma with multiple lymphomatous polyposis2014
Author(s)
Saito M, Miyazaki M, Tanino M, Tanaka S, Miyashita K, Izumiyama K, Mori A, Irie T, Tanaka M, Morioka M, Tsukamoto E.
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Journal Title
World J Gastroenterol
Volume: 20
Pages: 5141-5146
DOI
Related Report
Peer Reviewed
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[Journal Article] Pathology of frontotemporal dementia with limb girdle muscular dystrophy caused by a DNAJB6 mutation.2014
Author(s)
Yabe I, Tanino M, Yaguchi H, Takiyama A, Cai H, Kanno H, Takahashi I, Hayashi Y, Watanabe M, Takahashi H, Hatakeyama S, Tanaka S, Sasaki H.
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Journal Title
Clinical Neurology and Neurosurgery
Volume: 127
Pages: 10-12
Related Report
Peer Reviewed
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[Journal Article] Co-Overexpression of GEP100 and AMAP1 Proteins Correlates with Rapid Local Recurrence after Breast Conservative Therapy.2013
Author(s)
Kinoshita R, Nam JM, Ito YM, Hatanaka KC, Hashimoto A, Handa H, Otsuka Y, Hashimoto S, Onodera Y, Hosoda M, Onodera S, Shimizu S, Tanaka S, Shirato H, Tanino M, Sabe H.
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Journal Title
PLoS One
Volume: 8
Pages: e76791
DOI
Related Report
Peer Reviewed
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[Journal Article] Downregulation of miRNA-31 induces taxane resistance in ovarian cancer cells through increase of receptor tyrosine kinase MET.2013
Author(s)
Mitamura T, Watari H, Wang L, Kanno H, Hassan MK, Miyazaki M, Katoh Y, Kimura T, Tanino M, Nishihara H, Tanaka S, Sakuragi N
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Journal Title
Oncogenesis
Volume: 2
Pages: e40
DOI
Related Report
Peer Reviewed
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[Journal Article] Relationship between Methyl CpG binding protein 2 and JC viral proteins2013
Author(s)
Takahashi K, Orba y, Kimura T, Wang L, Kohsaka S, Tsuada M, Tanino M, Nishiha H, Nagashima K, Sawa H, Tanaka S
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Journal Title
Jpn J Infect Dis
Volume: 66
Pages: 126-132
Related Report
Peer Reviewed
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[Journal Article] Intracranial mass-forming lesion associated with dural thickening and hypophysitis.2013
Author(s)
Kanno H, Tanino M, Watanabe K, Ozaki Y, Itoh T, Kimura T, Nishihara H, Itoh T, Narita T, Nagashima K, Tanaka S.
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Journal Title
Neuropathology
Volume: 33
Pages: 213-6
Related Report
Peer Reviewed
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[Journal Article] Large solid-pseudopapillary neoplasm of the pancreas with aberrant protein expression and mutation of β-catenin: a case report and literature review of the distribution of β-catenin mutation.2013
Author(s)
Kobayashi T, Ozasa M, Miyashita K, Saga A, Miwa K, Saito M, Morioka M, Takeuchi M, Takenouchi N, Yabiku T, Kanno H, Yuzawa S, Tanino M, Tanaka S, Kawakami H, Asaka M, Sakamoto N
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Journal Title
Intern Med
Volume: 52
Pages: 2051-6
Related Report
Peer Reviewed
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[Journal Article] Relationship between Methyl CpG binding protein 2 and JC viral proteins.2013
Author(s)
Kenta Takahashi, Yasuko Orba, Taichi Kimura, Lei Wang, Shinji Kohsaka, Masumi Tsuada, Mishie Tanino, Hiroshi Nishiha, Kazuo Nagashima, Hiromumi Sawa, Shinya Tanaka.
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Journal Title
Jpn J Infect Dis
Volume: 66
Pages: 126-132
Related Report
Peer Reviewed
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[Journal Article] Expression of O(6)-methylguanine DNA methyltransferase (MGMT) and immunohistochemical analysis of 12 pineal parenchymal tumors.2012
Author(s)
Kanno H, Nishihara H, Oikawa M, Ozaki Y, Murata J, Sawamura Y, Kato M, Kubota K, Tanino M, Kimura T, Nagashima K, Itoh T, Tanaka S.
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Journal Title
Neuropathology
Volume: 32
Pages: 647-53
DOI
Related Report
Peer Reviewed
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[Journal Article] Intracranial mass-forming lesion associated with dural thickening and hypophysitis.2012
Author(s)
Kanno, H., Tanino, M., Watanabe, K., Ozaki, Y., Itoh, T., Kimura, T., Nishihara, H., Itoh, T., Narita, T., Nagashima, K., Tanaka, S
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Journal Title
Neuropathology
Volume: 33
Pages: 213-216
Related Report
Peer Reviewed
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[Journal Article] A case of clear cell variant of solid-pseudopapillary tumor of the pancreas in an adult male patient.2012
Author(s)
Tanino, M.,Kohsaka, S.,Kimura, T.,Tabu, K.,Nishihara, H.,Sawa, H.,Kawami, H.,Kamada, H.,Shimizu, M. Tanaka, S.
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Journal Title
Ann Diagn Pathol
Volume: 16
Pages: 134-140
Related Report
Peer Reviewed
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[Presentation] メチル化遺伝子結合蛋白MeCP2のJCウイルス関連蛋白による転写制御の解析2012
Author(s)
高橋健太, 木村太一, 王磊, 高阪真路, 工藤伸一, 奴久妻聡一, 谷野美智枝, 西原広史, 澤洋文, 長嶋和郎, 田中伸哉
Organizer
第53回日本神経病理学会総会学術研究会
Place of Presentation
新潟コンベンションセンター
Year and Date
20120628-20120630
Related Report
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[Presentation] 右前頭葉に発生したのう胞性腫瘍2012
Author(s)
Tamio Ito, Shinya Tanaka, Yoshimaru Ozaki, Kenichi Sato, Mishie Tanino, Hirohiko Nakamura
Organizer
第30回日本脳腫瘍病理学会
Place of Presentation
名古屋国際会議場(名古屋)
Year and Date
20120524-20120526
Related Report
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