Expression cloning of the gene functioning in transformation of Caco-2 cells to M cells
| Project/Area Number |
24590410
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Human pathology
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| Research Institution | University of Fukui (2013-2014)
Shinshu University (2012) |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
KOJIMA Katsuhiko 信州大学, 医学部, 助教 (80345743)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | M細胞 / 発現クローニング / 粘膜免疫 / 炎症性腸疾患 |
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| Outline of Final Research Achievements |
Our research goal was to clone the gene that transforms Caco-2 cells into M cells, which transcytose antigenic molecules in the intestinal lumen to subepithelial immune cells, by employing the Caco-2/Raji cells co-culture system. Furthermore, we aimed to establish monoclonal antibodies recognizing the gene products to clarify changes in the number and distribution of M cells in inflammatory bowel diseases. However, due to the difficulty to obtain the optimal condition for the induction of M cell phenotype, we changed the strategy. We tried to establish monoclonal antibodies against GP2 and Spi-B, both of which are found to be markers for mouse M cells. We have not yet obtained the hybridoma, and currently, we are preparing obtaining optimal condition for hybridoma screening.
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Report
(4 results)
Research Products
(17 results)
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[Journal Article] Helicobacter pylori cholesteryl α-glucosides contribute to its pathogenicity and immune response by natural killer T cells2013
Author(s)
Ito Y, Vela JL, Matsumura F, Hoshino H, Tyznik A, Lee H, Girardi E, Zajonc DM, Liddington R, Kobayashi M, Bao X, Bugaytsova J, Boren T, Jin R, Zong Y, Seeberger PH, Nakayama J, Kronenberg M, Fukuda M
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Journal Title
PLoS One
Volume: 8
Issue: 12
Pages: e78191-e78191
DOI
Related Report
Peer Reviewed
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