| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Outline of Final Research Achievements |
53BP1 belongs to DNA damage response (DDR) molecules and rapidly localizes at the site of DNA double strand breaks (DSB) forming nuclear foci (NF). The presence of 53BP1 NF can be an indicator of endogenous DSBs reflecting GIN. We have reported that type of 53BP1 NF by immunofluorescence (IF) is useful to estimate GIN in diverse tumorigenesis. This study aimed to clarify the type of 53BP1 NF in thyroid follicular tumor (FT). A total of 69 thyroid tissues, including follicular adenomas (FA), minimally invasive follicular carcinomas (MFC) and widely invasive FC (WFC), was annalyzed in this study. The type of 53BP1 expression was classified as low DDR and high DDR types. In results, the number of high DDR type of 53BP1 NF in tumor cells appeared to significantly increase with progression of carcinogenesis, such as FA-MFC-WFC. The type of 53BP1 expression by IF seems to reflect GIN to estimate malignant potency of FT. This method may be available in preoperative diagnosis for FT.
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