| Project/Area Number |
24590419
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Human pathology
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| Research Institution | University of Miyazaki |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2014: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
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| Keywords | 肺癌 / LRP11 / HGF activator inhibitor / serine protease / HGF 肝細胞増殖因子 / LDLR-related protein 11 / 膜蛋白質 / HAI-1 / HGF |
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| Outline of Final Research Achievements |
There has been no literature reports about LRP11 in lung cancer. In Northern blot, the brain and prostate tissue and testis in human normal tissue was observed gene expression, mild gene expression was observed in the lung. Gene expression of LRP11 was detected by RT-PCR in lung cancer cell lines. To test anti humna-LRP11 antibodies, we made COS-hLRP11 cells which have expression of human LRP11 protein in COS-7 cells. Immunohistochemically, the cytoplasm and cell membrane of adenocarcinoma, squamous cell carcinoma and small cell carcinoma using surgical material (FFPE tissue) are positive for LRP11 using the anti-human LRP11 antibodies. Currently, we have search role of LRP11, especially relevance of clinocopathological findings and expression of LRP11, in lung cancer, using immunohistochemical study and real-time PCR.
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