Micro RNA expression in serrated pathway and its association with a potential target therapy
Project/Area Number |
24590429
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Human pathology
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Research Institution | Dokkyo Medical University |
Principal Investigator |
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Co-Investigator(Kenkyū-buntansha) |
SAITO Tsuyoshi 順天堂大学, 医学部人体病理病態学講座, 准教授 (80439736)
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Project Period (FY) |
2012-04-01 – 2015-03-31
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Project Status |
Completed (Fiscal Year 2014)
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Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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Keywords | マイクロRNA / 広基性鋸歯状腺腫/ポリープ / 鋸歯状発癌経路 / 大腸ポリープ / 遺伝子メチル化 / 発癌 / 大腸 / Wntシグナル / 広基性鋸歯状ポリープ / 鋸歯状腺癌 / 発癌経路 |
Outline of Final Research Achievements |
Sessile serrated adenoma/polyp (SSA/P) is considered as an early precursor in the serrated pathway leading to colorectal cancer development. To clarify WNT signaling activation and expression of microRNAs (20a/21/93/181b) in this pathway, we performed β-catenin immunostaining and methylation specific PCR for AXIN2, MCC and secreted frizzled-related proteins in SSA/Ps, SSA/Ps with high-grade dysplasia (SSA/P+HGD) and SSA/Ps with submucosal carcinoma (SSA/P+SM-Ca). Nuclear β-catenin immunolabelings and the methylation of AXIN2 or MCC showed stepwise increment from SSA/Ps through SSA/P+HGD to SSA/P+SM-Ca. A stepwise increment of miR21 and a stepwise decrement of miR20a/93/181b expressions were seen in this sequence. There were significant correlations of those miR expressions with AXIN2 or MCC methylation in this pathway. Our results point toward a potential target therapy of those miRs and methylated genes such as RNA-interference drug or demethylase in malignant progression of SSA/P.
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Report
(4 results)
Research Products
(30 results)
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[Journal Article] Protein expression and methylation of DNA repair genes hMLH1, hMSH2, MGMT and BRCA1 and their correlation with clinicopathological parameters and prognosis in basal-like breast cancer.2013
Author(s)
Alkam Y, Mitomi H, Nakai K, Himuro T, Saito T, Takahashi M, Arakawa A, Yao T, Saito M:
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Journal Title
Histopathology
Volume: 63
Issue: 5
Pages: 713-725
DOI
Related Report
Peer Reviewed
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[Presentation] Clinicopathologic characteristics and molecular associations of WNT/β-catenin signal in the serrated neoplasia pathway of the colorectum.2015
Author(s)
Murakami T, Mitomi H, Sakamoto N, Ritsuno H, Ueyama H, Matsumoto K, Shibuya T, Osada T, Yao T, Watanabe S
Organizer
Digestive Disease Week 2015
Place of Presentation
Washington DC, USA
Year and Date
2015-05-16 – 2015-05-19
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