| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Outline of Final Research Achievements |
Sessile serrated adenoma/polyp (SSA/P) is considered as an early precursor in the serrated pathway leading to colorectal cancer development. To clarify WNT signaling activation and expression of microRNAs (20a/21/93/181b) in this pathway, we performed β-catenin immunostaining and methylation specific PCR for AXIN2, MCC and secreted frizzled-related proteins in SSA/Ps, SSA/Ps with high-grade dysplasia (SSA/P+HGD) and SSA/Ps with submucosal carcinoma (SSA/P+SM-Ca). Nuclear β-catenin immunolabelings and the methylation of AXIN2 or MCC showed stepwise increment from SSA/Ps through SSA/P+HGD to SSA/P+SM-Ca. A stepwise increment of miR21 and a stepwise decrement of miR20a/93/181b expressions were seen in this sequence. There were significant correlations of those miR expressions with AXIN2 or MCC methylation in this pathway. Our results point toward a potential target therapy of those miRs and methylated genes such as RNA-interference drug or demethylase in malignant progression of SSA/P.
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