| Project/Area Number |
24590430
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Human pathology
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| Research Institution | Tokai University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
CARRERAS Joaquim 東海大学, 医学部, 講師 (90637191)
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| Research Collaborator |
KIKUTI Yara Yukie 東海大学, 医学部, 研究員
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 濾胞性リンパ腫 / 腫瘍関連ﾏｸﾛﾌｧｰｼ / cDNA microarray / Gene Set Enrichment解析 / 免疫組織化学 / Follicular lymphoma / GSEA / Follicular helper T-cell / TAM / dendritic cells / immunohistochemistry / 腫瘍関連マクロファージ / M1 macrophage / M2 macrophage / B細胞 / マクロファージ / T細胞 |
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| Outline of Final Research Achievements |
Out of 108 genes identified by gene set enrichment analysis and functional association network analysis for Follicular lymphoma (FL), we focused 4 molecules of BTLA for T-cells, HVEM for dendritic cells, FOXP3 for regulatory T-cells and PD-1 for follicular helper T-cells. We also retrieved 4 molecules as tumor associated macrophages (TAM) of CD68 for pan-macrophage, CD16 for M1-like, and MiTF & CD163 for M2-like. Immunohistochemistry using formalin-fixed paraffin-embedded sections of low grade FL (LG-FL), high grade FL (HG-FL), diffuse large B-cell lymphoma transformed from FL (tDLBCL）was performed. Each of CD68+ cells and MiTF+ cells was increased in HG-FL other than LG-FL. PD-1+ cells was decreased in transformed DLBCL compared to LG-FL and HG-FL. Each of CD68+ cells and CD163+ cells was increased. Therefore, microenvironment including macrophages, dendritic cells and T-cells have important role for progression of FL and transformation to DLBCL.
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