| Project/Area Number |
24590445
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Human pathology
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| Research Institution | Hamamatsu University School of Medicine |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 超音波顕微鏡 / 組織弾性 / 音速 / 減衰 / 組織化学 / 架橋反応 / 加齢 / 細胞診 / イメージング / 弾性 / 組織診断 / 化学修飾 / 超音波 / 顕微鏡 / imaging analyssis / ホルマリン固定 / collagenase / pepsin / PAS反応 / 線維化 / 肉芽腫 / 壊死 / 硬癌 / 髄様癌 / デジタルイメージング |
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| Outline of Final Research Achievements |
To increase the sensitivity of imaging with a scanning acoustic microscope, we compared the difference of fixation method. By formalin fixation, cells or tissues shrunk and the speed-of-sound (SOS) and the attenuation-of-sound of fixation areas increased. From 3 min through 1 day of fixation period, the SOS rose accordingly. In routine formalin-fixed, paraffin-embedded section, no differences were found among fixation period from 1 day through 3 months.
To obtain specific images, colloidal gold fixed antibody or Ni-DAB staining method improved the positive images by increasing SOS. With periodic acid-Schiff (PAS) reaction, positive area showed greater SOS in proportion to the degree of glycosylation. By protease digestion, we could followed the time course of tissue breakdown.
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