Establishment of early diagnosis of pabcreatobiliary neoplasm

• Project/Area Number: 24590447
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Human pathology
• Research Institution: Kagoshima University
• Principal Investigator: HIGASHI Michiyo 鹿児島大学, 医歯(薬)学総合研究科, 准教授 (60315405)
• Co-Investigator(Kenkyū-buntansha): YONEZAWA Suguru 鹿児島大学, 大学院医歯学総合研究科, 教授 (10175002) ; YOKOYAMA Seiya 鹿児島大学, 大学院医歯学総合研究科, 助教 (20569941)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2014: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 病理学 / 膵胆道腫瘍 / ムチン抗原 / MUC1 / MUC4 / MUC16 / 早期診断 / 予後因子 / 膵胆管系腫瘍 / 予後

Outline of Final Research Achievements

We had reported that expression profiles of mucins are closely associated with biological behavior of human pancreatobiliary neoplasms. We tried to analyse of clinical human samples, especially pancreatic juices used by a new method, MSE (methylation specific analysis) developed by us. This new tool has a potential for the application to possibility of distinguishing tumor between the pancreatic tumors with better prognosis or worse prognosis. In addition, we have found that expression profiles of mucin in EUS-FNA specimens are associated with biological behavior. Thus, analyses of mucin expression in pancreatic juice or EUS-FNA specimens are useful for pathological diagnosis and/or patients' treatment.

Research Products

• [Journal Article] Combination of MUC1 and MUC4 expression predicts clinical outcome in patients with oral squamous cell carcinoma (2015)
  • Author(s): Kamikawa, Y. Kanmura, Y. Hamada, T. Yamada, N. Macha, M. A. Batra, S. K. Higashi, M. Yonezawa, S. Sugihara, K.
  • Journal Title: Int J Clin Oncol Volume: 20 Issue: 2 Pages: 298-307
  • DOI: 10.1007/s10147-014-0710-6
  • Peer Reviewed
• [Journal Article] 新規DNAメチル化解析法の開発と応用 (2015)
  • Author(s): 横山勢也、北本祥、山田宗茂、東美智代、米澤傑
  • Journal Title: 化学工業 Volume: 66 Pages: 28-33
  • Acknowledgement Compliant
• [Journal Article] Contrast-enhanced CT and diffusion-weighted MR imaging: Performance as a prognostic factor in patients with pancreatic ductal adenocarcinoma (2014)
  • Author(s): Fukukura, Y. Takumi, K. Higashi, M. Shinchi, H. Kamimura, K. Yoneyama, T. Tateyama, A.
  • Journal Title: Eur J Radiol Volume: 83 Issue: 4 Pages: 612-619
  • DOI: 10.1016/j.ejrad.2013.12.016
  • Peer Reviewed / Open Access
• [Journal Article] Pathobiological implications of mucin (MUC) expression in the outcome of small bowel cancer (2014)
  • Author(s): Shibahara H, Higashi M, Koriyama C, Yokoyama S, Kitazono I, Yasuhiro K, Narita M, Kuze S, Takanori K, Mita S, Arai T, Kato T, Yuasa N, Yamaguchi R, Kubota H, Suzuki H, Baba S, Rousseau K, Batra S K, Yonezawa S
  • Journal Title: PLoS ONE Volume: 9(4) Issue: 4 Pages: e86111-e86111
  • DOI: 10.1371/journal.pone.0086111
  • Peer Reviewed / Open Access
• [Journal Article] A comprehensive expression analysis of mucins in appendiceal carcinoma in a multicenter study: MUC3 is a novel prognostic factor (2014)
  • Author(s): Shibahara, H. Higashi, M. Yokoyama, S. Rousseau, K. Kitazono, I. Osako, M. Shirahama, H. Tashiro, Y. Kurumiya, Y. Narita, M. Kuze, S. Hasagawa, H. et al.
  • Journal Title: PLoS One Volume: 9 Issue: 12 Pages: e115613-e115613
  • DOI: 10.1371/journal.pone.0115613
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Diagnosis of pancreatic neoplasms using a novel method of DNA methylation analysis of mucin expression in pancreatic juice (2014)
  • Author(s): Yokoyama S, Kitamoto S, Higashi M, Goto Y, Hara T, Ikebe D, Yamaguchi T, Arisaka Y, Niihara, Nishimata H, Tanaka S, Takaori K, Batra S K, Yonezawa S
  • Journal Title: PLoS ONE Volume: 9(4) Issue: 4 Pages: e93760-e93760
  • DOI: 10.1371/journal.pone.0093760
  • Peer Reviewed / Open Access
• [Journal Article] MUC1 enhances hypoxia-driven angiogenesis through the regulation of multiple proangiogenic factors (2013)
  • Author(s): Sho Kitamoto, Soiya Yokoyama, Michiyo Higashi, Norishlge Yamada, Sonshin Takao, Suguru Yonezawa
  • Journal Title: Oncogene Volume: 32(39) Issue: 39 Pages: 4614-4621
  • DOI: 10.1038/onc.2012.478
  • Peer Reviewed
• [Journal Article] Expression of MUC4 mucin is observed mainly in the intestinal-type of intraductal papillary mucinous neoplasm of the pancreas (2013)
  • Author(s): Kitazono I, Higashi M, Kitamoto S, Yokoyama S, Horinouchi M, Osako M, Shimizu T, Tabata M, Batra S K, Goto M, Yonezawa S
  • Journal Title: Pancreas Volume: 42(7) Issue: 7 Pages: 1120-1128
  • DOI: 10.1097/mpa.0b013e3182965915
  • Peer Reviewed
• [Journal Article] 術前化学(放射線)療法を行った切除可能および境界膵癌の検討 (2013)
  • Author(s): 又木 雄弘、新地 洋之、前村 公成、蔵原 弘、川崎 洋太、出先 亮介、上野 真一、迫田 雅彦、 飯野 聡 、東 美智代、 高尾 尊身、夏越 祥次
  • Journal Title: 癌と化学療法 Volume: 40 Pages: 1637-1640
  • Peer Reviewed
• [Journal Article] 胆道系腫瘍における粘液産生の組織学的側面 (2013)
  • Author(s): 東 美智代、 柴原 弘明、 後藤 優子、 平木 翼、 横山 勢也、 米澤 傑
  • Journal Title: 胆と膵 Volume: 34 Pages: 353-358
• [Journal Article] 胆膵共通疾患とトピックス 膵胆道腫瘍におけるMUC発現 (2013)
  • Author(s): 米澤 傑、 東 美智代、 横山 勢也、 後藤 優子、北島 信一
  • Journal Title: 病理と臨床 Volume: 31 Pages: 399-408
• [Journal Article] MUC4 and MUC1 Expression in Adenocarcinoma of the Stomach Correlates with Vessel Invasion and Lymph Node Metastasis : An Immunohisto chemical Study of Early Gastric Cancer. (2013)
  • Author(s): Yukihiro Tamura, Michiyo Higashi, Sho Kitamoto, Seiya Yokoyama, Masahiko Osako, Michiko Horinouchi, Takeshi Shimizu, Mineo Tabata, Surinder K. Batra, Masamichi Goto, Suguru Yonezawa
  • Journal Title: PLoS ONE Volume: (in press) Issue: 11 Pages: e49251-e49251
  • DOI: 10.1371/journal.pone.0049251
  • Peer Reviewed
• [Journal Article] The etiologic role of human papillomavirus in penile cancers: a study in Vietnam (2013)
  • Author(s): Do, H. T. Koriyama, C. Khan, N. A. Higashi, M. Kato, T. Le, N. T. Matsushita, S. Kanekura, T. Akiba, S.
  • Journal Title: Br J Cancer Volume: 108 Issue: 1 Pages: 229-233
  • DOI: 10.1038/bjc.2012.583
  • Peer Reviewed
• [Journal Article] Pathobiological implications of expression of MUC16/CA125 in intrahepatic cholangiocarcinoma-mass formina type (2012)
  • Author(s): Michiyo Higashi
  • Journal Title: Pathobiology Volume: 79 Issue: 2 Pages: 101-106
  • DOI: 10.1159/000335164
  • Peer Reviewed
• [Journal Article] The application of Methylation specific electrophoresis (MSE) to DNA methylation analysis of the 5' CpG island of mucin in cancer cells (2012)
  • Author(s): Seiya Yokoyama
  • Journal Title: BMC Cancer Volume: 12(In press) Issue: 1 Pages: 67-67
  • DOI: 10.1186/1471-2407-12-67
  • Peer Reviewed
• [Journal Article] A novel anti-MUC1 antibody against the MUC1 cytoplasmic tail domain : use in sensitive identification of poorly differentiated cells in adenocarcinoma of the stomach (2012)
  • Author(s): Suguru Yonezawa
  • Journal Title: Gastric Cancer Volume: (In press) Issue: 4 Pages: 370-381
  • DOI: 10.1007/s10120-011-0125-2
  • Peer Reviewed
• [Presentation] 膵漿液性腫瘍の病理 (2015)
  • Author(s): 東美智代
  • Organizer: 日本病理学会
  • Place of Presentation: 名古屋(名古屋国際会議場)
  • Year and Date: 2015-04-30 – 2015-05-02
• [Presentation] EUS-FNA検体を用いたムチン染色有用性の検討 (2014)
  • Author(s): 東美智代、畑中一仁、竹下かおり、西田ゆかり、舞木公子、田中和彦、北島信一
  • Organizer: 臨床細胞学会
  • Place of Presentation: 神奈川(パシフィコ横浜)
  • Year and Date: 2014-06-05 – 2014-06-07
• [Presentation] IPMNにおけるムチン発現: 組織亜型との関連性について (2013)
  • Author(s): 米澤 傑
  • Organizer: 千葉胆膵疾患講演会
  • Place of Presentation: 京成ホテルミラマーレ(千葉)
  • Invited
• [Presentation] Mucin expression in pancreatic ductal adenocarcinoma: Immunohistochemical examination in EUS-FNAB specimens (2013)
  • Author(s): Michiyo Higashi, Yuko Goto, Suguru Yonezawa
  • Organizer: 25th European Congress of Pathology
  • Place of Presentation: Centro de Congressos de Lisboa (Lisbon, Portugal)
• [Presentation] Application of a novel DNA methylation analysis method (MSE) for mucin expression in pancreatic, biliary, and pulmonary neoplasms (2013)
  • Author(s): Suguru Yonezawa, Seiya Yokoyama, Sho Kitamoto, Michiyo Higashi
  • Organizer: MUCINS IN HEALTH AND DISEASE (12th International Workshop on Carcinoma-associated Mucins)
  • Place of Presentation: Robinson College (Cambridge, UK)
• [Presentation] Diagnosis of pancreatic neoplasms by application of a novel DNA methylation analysis method (MSE) for mucin expression in pancreatic juice (2013)
  • Author(s): Suguru Yonezawa, Seiya Yokoyama, Michiyo Higashi, Yuko Goto, Shinichi Kitajima
  • Organizer: Worldwide Innovate Networking in personalized cancer medicine
  • Place of Presentation: Le Palais des Congred (Paris, France)
• [Presentation] 膵癌におけるMUC1遺伝子のメチル化ステータスとmRNA発現解析 (2013)
  • Author(s): 横山勢也、北本祥、堤田英明、脇本譲二、東美智代、米澤傑
  • Organizer: 第102回日本病理学会総会
  • Place of Presentation: ロイトン札幌(北海道)
• [Presentation] 浸潤性膵管癌におけるEUS-FNABでのムチン発現 (2013)
  • Author(s): 東美智代、横山勢也、後藤優子、平木翼、後藤正道、米澤傑
  • Organizer: 第102回日本病理学会総会
  • Place of Presentation: ロイトン札幌(北海道)
• [Presentation] 膵内分泌腫瘍におけるMUC17発現の検討 (2012)
  • Author(s): 東美智代、北本祥、横山勢也、田畑和宏、北薗育美、平木翼、米澤傑
  • Organizer: 日本病理学会総会
  • Place of Presentation: 東京
• [Presentation] 胆道の前癌病変について 粘液形質の観点から (2012)
  • Author(s): 東美智代
  • Organizer: 胆道学会
  • Place of Presentation: 東京
  • Invited
• [Book] 専門医のための消化器病学第２版(小俣政男、千葉勉監修)、膵腫瘍におけるムチンコア蛋白(MUC)の分子病理(分担) (2013)
  • Author(s): 米澤傑、東美智代
  • Publisher: 医学書院
• [Remarks] 鹿児島大学大学院医歯学総合研究科先進治療科学専攻腫瘍学講座人体がん病理学(旧第二病理)
  • URL: http://www.kufm.kagoshima-u.ac.jp/~byouri2/