| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Outline of Final Research Achievements |
In this research period, we isolated cancer stem-like cells(CSCs)/cancer-initiating cells(CICs) from human lung, colon and kidney cancer line cells. To identify CSC/CIC-specific genes, we performed cDNA microarray analysis and found that numbers of cancer-testis (CT) antigens including OR7C1, DNAJB8 and SMCP were preferentially expressed in CSCs/CICs. Furthremore, we identified HLA-A24 restricted antigenic peptides encoded by amino acid sequences of OR7C1 and DNAJB8. Cytotoxic T lymphocytes (CTLs) specific for OR7C1 peptide and DNAJB8 peptide showed preferential cytotoxicity for CSCs/CICs compared with that of non-CSCs/CICs. These observations indicate that CT antigens-specific cancer immunotherapy might be promising approach for targeting treatment resistant CSCs/CICs.
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