Immunological analysis of treatment resistant cancer stem cells
Project/Area Number |
24590449
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Human pathology
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Research Institution | Sapporo Medical University |
Principal Investigator |
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Project Period (FY) |
2012-04-01 – 2015-03-31
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Project Status |
Completed (Fiscal Year 2014)
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Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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Keywords | がん幹細胞 / 免疫療法 / がん抗原 / がん精巣抗原 / DNAJB8 / OR7C1 / 大腸がん / 腫瘍抗原 / 抗原分子 |
Outline of Final Research Achievements |
In this research period, we isolated cancer stem-like cells(CSCs)/cancer-initiating cells(CICs) from human lung, colon and kidney cancer line cells. To identify CSC/CIC-specific genes, we performed cDNA microarray analysis and found that numbers of cancer-testis (CT) antigens including OR7C1, DNAJB8 and SMCP were preferentially expressed in CSCs/CICs. Furthremore, we identified HLA-A24 restricted antigenic peptides encoded by amino acid sequences of OR7C1 and DNAJB8. Cytotoxic T lymphocytes (CTLs) specific for OR7C1 peptide and DNAJB8 peptide showed preferential cytotoxicity for CSCs/CICs compared with that of non-CSCs/CICs. These observations indicate that CT antigens-specific cancer immunotherapy might be promising approach for targeting treatment resistant CSCs/CICs.
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Report
(4 results)
Research Products
(23 results)
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[Journal Article] Heat shock protein DNAJB8 is a novel target for immunotherapy of colon cancer-initiating cells.2014
Author(s)
Morita R, Nishizawa S, Torigoe T, Takahashi A, Tamura Y, Tsukahara T, Kanaseki T, Sokolovskaya A, Kochin V, Kondo T, Hashino S, Asaka M, Hara I, Hirohashi Y, Sato N.
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Journal Title
Cancer Sci
Volume: 105
Issue: 4
Pages: 389-395
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Fibroblasts induce expression of FGF4 in ovarian cancer stem-like cells/cancer-initiating cells and upregulate their tumor initiation capacity.2014
Author(s)
Yasuda K, Torigoe T, Mariya T, Asano T, Kuroda T, Matsuzaki J, Ikeda K, Yamauchi M, Emori M, Asanuma H, Hasegawa T, Saito T, Hirohashi Y, Sato N.
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Journal Title
Lab Invest
Volume: 94
Issue: 12
Pages: 1355-69
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Ectopically expressed variant form of sperm mitochondria-associated cysteine-rich protein augments tumorigenicity of the stem cell population of lung adenocarcinoma cells.2013
Author(s)
Takahashi A, Hirohashi Y, Torigoe T, Tamura Y, Tsukahara T, Kanaseki T, Kochin V, Saijo H, Kubo T, Nakatsugawa M, Asanuma H, Hasegawa T, Kondo T, Sato N.
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Journal Title
PLoS One
Volume: 8
Issue: 11
Pages: e69095-e69095
DOI
Related Report
Peer Reviewed
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[Journal Article] Ovarian cancer stem cells are enriched in side population and aldehyde dehydrogenase bright overlapping population.2013
Author(s)
Yasuda K, Torigoe T, Morita R, Kuroda T, Takahashi A, Matsuzaki J, Kochin V, Asanuma H, Hasegawa T, Saito T, Hirohashi Y, Sato N.
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Journal Title
PLoS One
Volume: 8(8)
Issue: 8
Pages: e68187-e68187
DOI
Related Report
Peer Reviewed
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[Journal Article] Small proline-rich protein-1B is overexpressed in human oral squamous cell cancer stem-like cells and is related to their growth through activation of MAP kinase signal.2013
Author(s)
Michifuri Y, Hirohashi Y, Torigoe T, Miyazaki A, Fujino J, Tamura Y, Tsukahara T, Kanaseki T, Kobayashi J, Sasaki T, Takahashi A, Nakamori K, Yamaguchi A, Hiratsuka H, Sato N.
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Journal Title
Biochem Biophys Res Commun
Volume: 439
Issue: 1
Pages: 96-102
DOI
Related Report
Peer Reviewed
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[Journal Article] Preferential expression of cancer/testis genes in cancer stem-like cells : proposal of a novel sub-category, cancer/testis/stem gene.2013
Author(s)
Yamada R, Takahashi A, Torigoe T, Morita R, Tamura Y, Tsukahara T, Kanaseki T, Kubo T, Watarai K, Kondo T, Hirohashi Y, Sato N.
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Journal Title
Tissue Antigens.
Volume: 81(6)
Issue: 6
Pages: 428-34
DOI
Related Report
Peer Reviewed
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[Journal Article] Prostate cancer stem-like cells/cancer-initiating cells have an autocrine system of hepatocyte growth factor.2013
Author(s)
Nishida S, Hirohashi Y, Torigoe T, Inoue R, Kitamura H, Tanaka T, Takahashi A, Asanuma H, Masumori N, Tsukamoto T, Sato N.
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Journal Title
Cancer Sci
Volume: 104
Issue: 4
Pages: 431-6
DOI
Related Report
Peer Reviewed
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