| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
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| Outline of Final Research Achievements |
We evaluated the biological significance of DNA methylation in the regulation of drug metabolizing enzyme (DME) genes by genome-wide integrative analysis. Among normal livers, 7 DME genes showed significant inverse correlations between DNA methylation and mRNA expression. In hepatoma cells, treatment with a demethylating agent resulted in upregulation of 5 DME genes, which could be explained by DNA methylation status. Tissue-specific and age-dependent expression of UDP-glucuronosyl transferase 1A splicing variants could be explained by DNA methylation status. Interestingly, some DME genes had unique methylation features similar to those observed in tumor-suppressor or housekeeping genes. Analysis of the DNA methylation landscape facilitated elucidation of the role of DNA methylation.
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