Expression analysis of hWAPL oncogene

Research Project

Project/Area Number	24590453
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Multi-year Fund
Section	一般
Research Field	Human pathology
Research Institution	Tokyo Medical University
Principal Investigator	YAMADA Masatoshi 東京医科大学, 医学部, 助教 (50449120)
Co-Investigator(Kenkyū-buntansha)	KURODA Masahiko 東京医科大学, 医学部, 教授 (80251304) KURATA Atsushi 東京医科大学, 医学部, 准教授 (10302689)
Project Period (FY)	2012-04-01 – 2015-03-31
Project Status	Completed (Fiscal Year 2014)
Budget Amount *help	¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000) Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000) Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000) Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywords	hWAPL / 子宮頸部病変 / HPV / ヒトパピローマウイルス / 国内研究
Outline of Final Research Achievements	By use of immunohistochemical method, we showed that the expression of hWAPL protein increases in the cervical lesion. The expression of hWAPL protein increased as the cervical lesion progresses from mild dysplasia (CIN1) to severe dysplasia (CIN3) and carcinoma. This indicates that hWAPL protein is important to form cervical lesions. We also demonstrated that the detection of mRNA of human papilloma virus (HPV), which is associated with cervical lesions and the expression of hWAPL, is more specific than that of DNA.