| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Outline of Final Research Achievements |
Dendritic cells are professional antigen presenting cells and consist of several subsets with specialized functions. A XC chemokine receptor 1 (XCR1) is selectively expressed on a dendritic cell subset showing a high ability to cross-present antigens to naive CD8 T cells. Conditional ablation of these XCR1+ dendritic cells in vivo are achieved by generation of gene modified mice in which the XCR1 gene was replaced with a gene coding for a fusion protein consisting of human diphtheria toxin receptor and fluorescent protein venus. In these mice, XCR1+ dendritic cells were transiently and conditionally ablated when diphtheria toxin was injected. Using this system, we found that cross-presentation as well as oral tolerance was impaired in the absence of XCR1+ dendritic cells in vivo. These results suggest that XCR1+ dendritic cells play important roles in induction of not only immune response but also immune tolerance.
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