Analysis of molecular mechanism of oxidative stress regulation by RNA splicing
| Project/Area Number |
24590464
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Experimental pathology
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| Research Institution | Tokai University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
NAKAMURA Naoya 東海大学, 医学部, 教授 (50227922)
TAKIZAWA Shunya 東海大学, 医学部, 教授 (70197234)
HORIKOSHI Yosuke 東海大学, 医学部, 研究員 (60448678)
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| Project Period (FY) |
2012-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2015: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2014: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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| Keywords | 酸化ストレス / RNAスプライシング / シグナル伝達 / protein kinase c / RNA splicing / oxidative stress / diacylglycerol / プロテインキナーゼC / ジアシルグリセロール / スプライシング / PKC |
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| Outline of Final Research Achievements |
Diacylglycerol (DAG) is a physiological activator of protein kinase C (PKC). We reported that oxidized diacylglycerol (DAG-OOH) had a remarkably stronger PKC-activating action than un-oxidized DAG. DAG-OOH also induces neuronal cell injury through the over-activation of PKC delta molecule. On the other hand, PKC delta splicing variant (PKCSV) could prevent DAG-OOH-induced neuronal cell injury. In this study, the action of PKCSV to prevent oxidative stress- induced neuronal cell injury was analyzed and the expression of PKCSV under various oxidative stress arising was observed. In consequence, PKCSV over-expressing neuronal cells resistance to cell injury evoked by superoxide radical, hydrogen peroxide and DAG-OOH. Furthermore the expression of PKCSV significantly increased in oxidative stress models These result suggested that PKCSV has a protective action against oxidative stress-induce cell injury and the cell defense mechanism through the RNA splicing may exist.
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Report
(5 results)
Research Products
(15 results)
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[Journal Article] Localization of aPKC Lambda/Iota and Its Interacting Protein, Lgl2, Is Significantly Associated with Lung Adenocarcinoma Progression.2013
Author(s)
Imamura N, Horikoshi Y, Matsuzaki T, Toriumi K, Kitatani K, Ogura G, Masuda R, Nakamura N, Takekoshi S, Iwazaki M
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Journal Title
Tokai J Exp Clin Med.
Volume: 38
Pages: 146-158
Related Report
Peer Reviewed
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[Journal Article] Over-expression of BACH2 is related to ongoing somatic hypermutation of the immunoglobulin heavy chain gene variable region of de novo diffuse large B-cell lymphoma.2013
Author(s)
Kikuchi T, Tokunaka M, Kikuti YY, Carreras J, Ogura G, Takekoshi S, Kojima M, Ando K, Hashimoto Y, Abe M, Takata K, Yoshino T, Muto A, Igarashi K, Nakamura N.
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Journal Title
Pathol Int.
Volume: 63
Pages: 339-344
Related Report
Peer Reviewed
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