| Project/Area Number |
24590464
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Experimental pathology
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| Research Institution | Tokai University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
NAKAMURA Naoya 東海大学, 医学部, 教授 (50227922)
TAKIZAWA Shunya 東海大学, 医学部, 教授 (70197234)
HORIKOSHI Yosuke 東海大学, 医学部, 研究員 (60448678)
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| Project Period (FY) |
2012-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2015: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2014: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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| Keywords | 酸化ストレス / RNAスプライシング / シグナル伝達 / protein kinase c / RNA splicing / oxidative stress / diacylglycerol / プロテインキナーゼC / ジアシルグリセロール / スプライシング / PKC |
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| Outline of Final Research Achievements |
Diacylglycerol (DAG) is a physiological activator of protein kinase C (PKC). We reported that oxidized diacylglycerol (DAG-OOH) had a remarkably stronger PKC-activating action than un-oxidized DAG. DAG-OOH also induces neuronal cell injury through the over-activation of PKC delta molecule. On the other hand, PKC delta splicing variant (PKCSV) could prevent DAG-OOH-induced neuronal cell injury. In this study, the action of PKCSV to prevent oxidative stress- induced neuronal cell injury was analyzed and the expression of PKCSV under various oxidative stress arising was observed. In consequence, PKCSV over-expressing neuronal cells resistance to cell injury evoked by superoxide radical, hydrogen peroxide and DAG-OOH. Furthermore the expression of PKCSV significantly increased in oxidative stress models These result suggested that PKCSV has a protective action against oxidative stress-induce cell injury and the cell defense mechanism through the RNA splicing may exist.
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