Development of DNA demethylation technology in cancer cells and its application for discovering biomarkers for early diagnosis
| Project/Area Number |
24590471
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Experimental pathology
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| Research Institution | Tohoku University |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 腫瘍 / DNAメチル化 / DNA脱メチル化 / 癌 / ゲノム / 遺伝子 / 発現制御 / 脱メチル化 / 転写活性化 |
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| Outline of Final Research Achievements |
We have developed a novel method that globally demethylates and reactivates hypermethylated genes by using a fusion gene comprising of methyl-CpG binding domain (MBD) and the catalytic domain of Ten-eleven translocation protein 1 (TET1-CD). This method is useful for identifying hypermethylation-mediated silenced genes in cancer cells. We have applied it to prostate and colorectal cancer cell lines and found that DNA demethylation suppressed the growth of cancer cells. These results suggest that DNA methylation confers the ability of cancer cells to facilitate the growth and DNA methylation of gene(s) responsible for cell growth may be useful as a biomarker.
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Report
(4 results)
Research Products
(22 results)
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[Journal Article] S100A4, frequently overexpressed in various human cancers, accelerates cell motility in pancreatic cancercells2012
Author(s)
Sekine H, Chen N, Sato K, Saiki Y, Yoshino Y, Umetsu Y, Jin G, Nagase H, Gu Z, Fukushige S, Sunamura M, Horii A.
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Journal Title
Biochem Biophys Res Commun
Volume: 429
Issue: 3-4
Pages: 214-219
DOI
Related Report
Peer Reviewed
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[Presentation] IFI27 and NOV, downstream regulated genes by S100A4, are playing important roles in pancreatic carcinogenesis.2014
Author(s)
Chen N, Saiki Y, Sekine H, Sunamura M, Fukushige S, Motoi F, Egawa S, Unno M, Hroii A
Organizer
105th American Association for Cancer Research Annual Meeting
Place of Presentation
San Diego, CA, USA
Year and Date
2014-04-05 – 2014-04-09
Related Report
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