| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Outline of Final Research Achievements |
To make clear roles of REV7 in spermatogenesis in the testis, we tried to generate Rev7 conditional knockout mouse, in which Rev7 could be deleted in the adult period. Because we could not generate the mouse in our first plan, we purchased commercially-available gene targeted mouse and are now generating the Rev7 conditional knockout mouse. We performed another project to identify REV7-interacting proteins in the testis using Rev7 transgenic mouse testes. Although several candidate bands were detected in the immunoprecipitation analysis, we could not analyze them by mass spectrometry because of less amount of the proteins. We will continue the project in the future.
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