Degenerative therapy for heart failure using autologous myocardial fibroblast.
| Project/Area Number |
24590482
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Experimental pathology
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| Research Institution | Osaka University |
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Principal Investigator |
KAWAGUCHI Naomasa 大阪大学, 医学(系)研究科(研究院), 准教授 (70224748)
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| Co-Investigator(Kenkyū-buntansha) |
MORI Seiji 大阪大学, 大学院医学系研究科, 准教授 (90467506)
HAMADA Yoshinosuke 大阪大学, 大学院医学系研究科, 准教授 (10362683)
MATSUURA Nariaki 大阪大学, 大学院医学系研究科, 教授 (70190402)
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| Co-Investigator(Renkei-kenkyūsha) |
MIYAGAWA Sigeru 大阪大学, 大学院医学系研究科, 准教授 (70544237)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 再生医学 / 再生医療 |
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| Outline of Final Research Achievements |
We examined the effects of tissue inhibitors of metalloproteinases (TIMPs) on cardiac fibroblasts (CFs) and cardiomyocytes(CM). In vitro, TIMP-1～-4 enhanced smooth muscle actin (SMA) expression in CFs, and TIMP-1 and TIMP-3 enhanced the expression of phosphorylated Smad-3 and phosphorylated transforming growth factor β type 1 receptor in CFs. TIMP-1, -3, and -4 also inhibited the FAK, AKT, and ERK pathways that induce cardiac hypertrophy. Collagen gels containing TIMP-1 or TIMP-3 were transplanted to the left ventricular anterior wall of an ischemic cardiomyopathy(ICM) rat. Gel-released TIMP-1 and TIMP-3 significantly improved cardiac function and myocardial remodeling and enhanced SMA expression in the infarcted area in ICM rat. TIMPs may be an ideal target of cardiac regeneration therapy.
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Report
(4 results)
Research Products
(7 results)
