| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Outline of Final Research Achievements |
Previously, I have reported that miR-210 is constitutively upregulated in clear cell renal cell carcinoma because of the deregulation of VHL-HIF axis. To elucidate whether the upregulation of miR-210 is involved in the tumorigenesis and/or progression of renal cell carcinoma, I addressed the following two issues, 1) the identification of the target genes of miR-210 and 2) the observation of biological effect of miR-210 upregulation on renal tubules in vivo.
Research results; A miR-210 target gene which is conserved as target between human and mouse was identified by microarray analysis. MiR-210 transgenic mice which express miR-210 in renal proximal tubules were established and histopathologically analyzed. Tg-mice kept alive without specific symptoms and development of renal carcinoma at least for 96 weeks. The chronic inflammations were observed in kidneys of young Tg-mice, and their renal function was diminished with aging. The overexpression of miR-210 may damage the renal tubules.
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