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Involvement of lincRNA in carcinogenesis

Research Project

Project/Area Number 24590489
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Experimental pathology
Research InstitutionIwate Medical University

Principal Investigator

SHIBAZAKI Masahiko  岩手医科大学, 医学部, 助教 (20445109)

Co-Investigator(Kenkyū-buntansha) MAESAWA Chihaya  岩手医科大学, 大学院医学研究科, 教授 (10326647)
AKASAKA Toshihide  岩手医科大学, 大学院医学研究科, 教授 (30137525)
SUGIYAMA Toru  岩手医科大学, 大学院医学研究科, 教授 (40162903)
KASHIWABA Masahiro  岩手医科大学, 大学院医学研究科, 講師 (80326660)
Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Keywords非翻訳RNA / 腫瘍化 / lincRNA / 腫瘍 / 抗酸化関連分子
Outline of Final Research Achievements

In epithelial ovarian cancer cell lines and primary tumors, we investigated that miR-10b and/or HOTAIR can regulate the HOXD10 expression, and that it permit gain of pro-metastatic gene products. Our results suggested that downregulation of HOXD10 expression by miR-10b overexpression might induce increase of pro-metastatic gene products, such as MMP14 and RHOC, and contribute to the acquisition of metastatic phenotypes in epithelial ovarian cancer cells. Moreover, We identified a novel single-nucleotide deletion in codon 507 of exon 4 of the KEAP1 gene that resulted in a frameshift mutation in malignant melanoma. KEAP1-FSM cells exhibited significant resistance to hydrogen peroxide and anti-cancer agents such as cisplatin and dacarbazine, which are mostly used for melanoma chemotherapy. These data suggest that NRF2 may have potential value as a therapeutic target in malignant melanoma in order to improve the rate of clinical response to anti-cancer agents.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • 2012 Research-status Report
  • Research Products

    (18 results)

All 2014 2013 2012

All Journal Article (7 results) (of which Peer Reviewed: 7 results,  Open Access: 1 results) Presentation (11 results)

  • [Journal Article] Immunohistochemistry for histone h3 lysine 9 methyltransferase and demethylase proteins in human melanomas.2014

    • Author(s)
      Miura S, Maesawa C, Shibazaki M, Yasuhira S, Kasai S, Tsunoda K, Maeda F, Takahashi K, Akasaka T, Masuda T. Immunohistochemistry for histone h3 lysine 9 methyltransferase and demethylase proteins in human melanomas.
    • Journal Title

      Am J Dermatopathol

      Volume: 36(3) Issue: 3 Pages: 211-216

    • DOI

      10.1097/dad.0b013e3182964e02

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] A somatic mutation of the KEAP1 gene in malignant melanoma is involved in aberrant NRF2 activation and an increase in intrinsic drug resistance.2014

    • Author(s)
      Miura S, Shibazaki M, Kasai S, Yasuhira S, Watanabe A, Inoue T, Kageshita Y, Tsunoda K, Takahashi K, Akasaka T, Masuda T, Maesawa C.
    • Journal Title

      Journal of investigative dermatology

      Volume: 134 Pages: 553-556

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Immunohistochemistry for histone h3 lysine 9 methyltransferase and demethylase proteins in human melanomas.2014

    • Author(s)
      Miura S, Maesawa C, Shibazaki M, Yasuhira S, Kasai S, Tsunoda K, Maeda F, Takahashi K, Akasaka T, Masuda T.
    • Journal Title

      American journal of dermatopathology

      Volume: 36 Pages: 211-216

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Loss of HOXD10 expression induced by upregulation of miR-10b accelerates the migration and invasion activities of ovarian cancer cells.2013

    • Author(s)
      Nakayama I, Shibazaki M, Yashima-Abo A, Miura F, Sugiyama T, Masuda T, Maesawa C.
    • Journal Title

      International journal of oncology

      Volume: 43 Pages: 63-71

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] BCL2 and BCLxL are key determinants of resistance to antitubulin chemotherapeutics in melanoma cells.2013

    • Author(s)
      Watanabe A, Yasuhira S, Inoue T, Kasai S, Shibazaki M, Takahashi K, Akasaka T, Masuda T, Maesawa C.
    • Journal Title

      Experimental dermatology

      Volume: 22 Pages: 518-523

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Transcriptional and post-transcriptional regulation of βIII-tubulin protein expression in relation with cell cycle-dependent regulation of tumor cells2012

    • Author(s)
      Shibazaki M, Maesawa C, Akasaka K, Kasai S, Yasuhira S, Kanno K, Nakayama I, Sugiyama T, Wakabayasi G, Masuda T, Mori N
    • Journal Title

      Int.J.Oncol.

      Volume: 40(3) Pages: 695-702

    • DOI

      10.3892/ijo.2011.1291

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Downregulation of cylindromatosis gene, CYLD, confers a growth advantage on malignant melanoma cells while negatively regulating their migration activity2012

    • Author(s)
      Ishikawa Y, Tsunoda K, Shibazaki M, Takahashi K, Akasaka T, Masuda T, Maesawa C.
    • Journal Title

      Int J Oncol

      Volume: 41(1) Pages: 53-60

    • DOI

      10.3892/ijo.2012.1424

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Presentation] 悪性黒色腫におけるKEAP1遺伝子異変の意義2014

    • Author(s)
      前沢千早,柴崎晶彦,葛西秋宅,増田友之
    • Organizer
      第103回日本病理学会
    • Place of Presentation
      広島
    • Related Report
      2013 Research-status Report
  • [Presentation] microRNA-10bの発現増加によるHOXD10遺伝子の発現抑制は卵巣がん細胞における遊走・浸潤能を亢進させる2013

    • Author(s)
      中山育慧,柴崎晶彦,安平進士,葛西秋宅,杉山徹,増田友之,前沢千早
    • Organizer
      第102回日本病理学会
    • Place of Presentation
      札幌
    • Related Report
      2013 Research-status Report
  • [Presentation] 悪性黒色腫細胞株の網羅的エクソーム解析による新規関連遺伝子の検索2013

    • Author(s)
      柴崎晶彦,三浦慎平,安平進士,葛西秋宅,赤坂俊英,増田友之,前沢千早
    • Organizer
      第102回日本病理学会
    • Place of Presentation
      札幌
    • Related Report
      2013 Research-status Report
  • [Presentation] 悪性黒色腫におけるヒストンメチル化酸素、脱ヒストンメチル化酸素の発現の検討2013

    • Author(s)
      影下雄一,前沢千早,三浦慎平,柴崎晶彦,安平進士,葛西秋宅,角田加奈子,前田文彦,高橋和宏,赤坂俊英,増田友之
    • Organizer
      第102回日本病理学会
    • Place of Presentation
      札幌
    • Related Report
      2013 Research-status Report
  • [Presentation] 悪性黒色腫における抗チューブリン剤耐性はBCL2とBCL-XLによって規定される2013

    • Author(s)
      渡辺彩乃,安平進士,葛西秋宅,柴崎晶彦,赤坂俊英,増田友之,前沢千早
    • Organizer
      第102回日本病理学会
    • Place of Presentation
      札幌
    • Related Report
      2013 Research-status Report
  • [Presentation] 悪性黒色腫におけるCYLDによる細胞増殖、運動能の制御2012

    • Author(s)
      石川雄一,前沢千早,柴崎晶彦,安平進士,菅野公徳,及川浩樹,増田友之
    • Organizer
      第101回日本病理学会
    • Place of Presentation
      東京
    • Related Report
      2012 Research-status Report
  • [Presentation] 肝細胞癌培養細胞株におけるHGF/METシグナルに与える細胞質内ヒストン脱アセチル化酸素 HDAC6発現の影響2012

    • Author(s)
      菅野公徳,前沢千早,柴崎晶彦,安平進士,葛西秋宅,石川雄一,及川浩樹,増田友之
    • Organizer
      第101回日本病理学会
    • Place of Presentation
      東京
    • Related Report
      2012 Research-status Report
  • [Presentation] 悪性黒色腫においてCYLD遺伝子は増殖能獲得に関与するが運動能は抑制する2012

    • Author(s)
      石川雄一,前沢千早,角田加奈子,柴崎晶彦,赤坂俊英,増田友之
    • Organizer
      第71回日本癌学会学術総会
    • Place of Presentation
      札幌
    • Related Report
      2012 Research-status Report
  • [Presentation] 悪性黒色腫における抗チューブリン剤耐性はBCL2とBCL-XLによって規定される2012

    • Author(s)
      渡辺彩乃,安平進士,葛西秋宅,柴崎晶彦,赤坂俊英,増田友之,前沢千早
    • Organizer
      第35回日本分子生物学会年会
    • Place of Presentation
      福岡
    • Related Report
      2012 Research-status Report
  • [Presentation] 多能性維持関連因子NACC1(nucleus accumbens-associated protein 1)の細胞周期特異的リン酸化の制御2012

    • Author(s)
      葛西秋宅,加茂政晴,柴崎晶彦,安平進士,前沢千早
    • Organizer
      第35回日本分子生物学会年会
    • Place of Presentation
      福岡
    • Related Report
      2012 Research-status Report
  • [Presentation] 悪性黒色腫細胞株の網羅的エクソーム解析による新規関連遺伝子の検索2012

    • Author(s)
      柴崎晶彦,三浦慎平,安平進士,葛西秋宅,赤坂俊英,増田友之,前沢千早
    • Organizer
      第35回日本分子生物学会年会
    • Place of Presentation
      福岡
    • Related Report
      2012 Research-status Report

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Published: 2013-05-31   Modified: 2019-07-29  

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