Involvement of lincRNA in carcinogenesis
Project/Area Number |
24590489
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Experimental pathology
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Research Institution | Iwate Medical University |
Principal Investigator |
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Co-Investigator(Kenkyū-buntansha) |
MAESAWA Chihaya 岩手医科大学, 大学院医学研究科, 教授 (10326647)
AKASAKA Toshihide 岩手医科大学, 大学院医学研究科, 教授 (30137525)
SUGIYAMA Toru 岩手医科大学, 大学院医学研究科, 教授 (40162903)
KASHIWABA Masahiro 岩手医科大学, 大学院医学研究科, 講師 (80326660)
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Project Period (FY) |
2012-04-01 – 2015-03-31
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Project Status |
Completed (Fiscal Year 2014)
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Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
|
Keywords | 非翻訳RNA / 腫瘍化 / lincRNA / 腫瘍 / 抗酸化関連分子 |
Outline of Final Research Achievements |
In epithelial ovarian cancer cell lines and primary tumors, we investigated that miR-10b and/or HOTAIR can regulate the HOXD10 expression, and that it permit gain of pro-metastatic gene products. Our results suggested that downregulation of HOXD10 expression by miR-10b overexpression might induce increase of pro-metastatic gene products, such as MMP14 and RHOC, and contribute to the acquisition of metastatic phenotypes in epithelial ovarian cancer cells. Moreover, We identified a novel single-nucleotide deletion in codon 507 of exon 4 of the KEAP1 gene that resulted in a frameshift mutation in malignant melanoma. KEAP1-FSM cells exhibited significant resistance to hydrogen peroxide and anti-cancer agents such as cisplatin and dacarbazine, which are mostly used for melanoma chemotherapy. These data suggest that NRF2 may have potential value as a therapeutic target in malignant melanoma in order to improve the rate of clinical response to anti-cancer agents.
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Report
(4 results)
Research Products
(18 results)
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[Journal Article] A somatic mutation of the KEAP1 gene in malignant melanoma is involved in aberrant NRF2 activation and an increase in intrinsic drug resistance.2014
Author(s)
Miura S, Shibazaki M, Kasai S, Yasuhira S, Watanabe A, Inoue T, Kageshita Y, Tsunoda K, Takahashi K, Akasaka T, Masuda T, Maesawa C.
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Journal Title
Journal of investigative dermatology
Volume: 134
Pages: 553-556
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Peer Reviewed
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[Journal Article] Transcriptional and post-transcriptional regulation of βIII-tubulin protein expression in relation with cell cycle-dependent regulation of tumor cells2012
Author(s)
Shibazaki M, Maesawa C, Akasaka K, Kasai S, Yasuhira S, Kanno K, Nakayama I, Sugiyama T, Wakabayasi G, Masuda T, Mori N
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Journal Title
Int.J.Oncol.
Volume: 40(3)
Pages: 695-702
DOI
Related Report
Peer Reviewed
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[Presentation] 悪性黒色腫におけるヒストンメチル化酸素、脱ヒストンメチル化酸素の発現の検討2013
Author(s)
影下雄一,前沢千早,三浦慎平,柴崎晶彦,安平進士,葛西秋宅,角田加奈子,前田文彦,高橋和宏,赤坂俊英,増田友之
Organizer
第102回日本病理学会
Place of Presentation
札幌
Related Report
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