• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Research and development of human neutralizing antibodies aiming at treatment of non-small cell lung carcinoma

Research Project

Project/Area Number 24590490
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Experimental pathology
Research InstitutionKeio University

Principal Investigator

MOCHIZUKI Satsuki  慶應義塾大学, 医学部, 講師 (80365428)

Co-Investigator(Renkei-kenkyūsha) OKADA Yasunori  慶應義塾大学, 医学部, 教授 (00115221)
Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
KeywordsADAM / 分子標的 / 肺癌 / 癌転移 / 分子標的治療 / VWF / 癌細胞増殖 / ヒト型抗体
Outline of Final Research Achievements

ADAM28, a member of the ADAM (a disintegrin and metalloproteinase) gene family, plays an important role in cancer cell proliferation and metastasis. In the present study, we screened Human Combinatorial Antibody Library by phage display panning and obtained antibodies (211-14 and 211-12) against ADAM28. Tumor growth and metastasis of the lung tumors after intravenous injection of PC-9 ffLuc-cp156 lung carcinoma cells were significantly inhibited by the antibody treatment, improving survival rate of the mice. These data indicate that our antibodies 211-14 and 211-12 neutralize ADAM28 activity and antibody 211-14 inhibits cancer cell proliferation and metastasis in vivo. The antibodies could be used as an ADAM28 inhibitor for clinical application to treat the patients with lung or breast carcinoma in the future.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • 2012 Research-status Report
  • Research Products

    (25 results)

All 2015 2014 2013 2012 Other

All Journal Article (6 results) (of which Peer Reviewed: 6 results,  Open Access: 1 results) Presentation (15 results) Book (2 results) Remarks (2 results)

  • [Journal Article] CCN1 (Cyr61) is overexpressed in human osteoarthritic cartilage and inhibits ADAMTS4 (aggrecanase-1) activity2015

    • Author(s)
      Miyuki Chijiiwa, Satsuki Mochizuki, Tokuhiro Kimura, Hitoshi Abe, Yukie Tanaka,Yutaka Fujii, Hidenori Shimizu, Hiroyuki Enomoto, Yoshiaki Toyama and Yasunori Okada
    • Journal Title

      Arthritis Rheum

      Volume: in press Issue: 6 Pages: 1557-1567

    • DOI

      10.1002/art.39078

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Src plays a key role in ADAM28 expression in v-src-transformed epithelial cells and human carcinoma cells2013

    • Author(s)
      Abe H., Mochizuki S., Ohara K., Ueno M., Ochiai H., Kitagawa Y., Hino O., Sato H. and Okada Y.
    • Journal Title

      Am J Pathol

      Volume: 183 Issue: 5 Pages: 1667

    • DOI

      10.1016/j.ajpath.2013.07.011

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Effect of ADAM28 on Carcinoma Cell Metastasis by Cleavage of von Willebrand Factor2012

    • Author(s)
      Satsuki Mochizuki
    • Journal Title

      Journal of the National Cancer Institute

      Volume: 104(In press) Issue: 12 Pages: 906-922

    • DOI

      10.1093/jnci/djs232

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] ADAM28 is elevated in humans with the metabolic syndrome and is a novel sheddase of human tumour necrosis factor-α2012

    • Author(s)
      Jowett J.B.M., Okada Y., Leedman P.J., Curran J.E., Johnson M.P., Moses E.K., Goring H.H.H., Mochizuki S., Blangero J., Stone L., Allen H., Mitchell C. and Matthews V.B
    • Journal Title

      Immunol Cell Biol

      Volume: 90 Issue: 10 Pages: 966-973

    • DOI

      10.1038/icb.2012.44

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Soluble Vascular Adhesion Protein-1 Accumulates in Proliferative Diabetic Retinopathy.2012

    • Author(s)
      Murata M, Noda K, Fukuhara J, Kanda A, Kase S, Saito W, Ozawa Y, Mochizuki S, Kimura S, Mashima Y, Okada Y, Ishida S.
    • Journal Title

      Invest Ophthalmol Vis Sci.

      Volume: 53 Issue: 7 Pages: 4055-62

    • DOI

      10.1167/iovs.12-9857

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Regulatory Role of Dendritic Cells in Post-Infarction Healing and Left Ventricular Remodeling2012

    • Author(s)
      Anzai A, Fukuda K, et al
    • Journal Title

      Circulation

      Volume: 125(10) Issue: 10 Pages: 1234-1245

    • DOI

      10.1161/circulationaha.111.052126

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Presentation] 肺癌におけるADAM28を標的としたヒト型活性阻害抗体の開発2015

    • Author(s)
      望月早月
    • Organizer
      日本癌学会シンポジウム/共同利用・共同研究拠点シンポジウム
    • Place of Presentation
      金沢市、石川県、石川県立音楽堂交流ホール
    • Year and Date
      2015-01-21 – 2015-01-22
    • Related Report
      2014 Annual Research Report
  • [Presentation] Research and development of human neutralizing antibodies aiming at treatment of non-small cell lung carcinoma2014

    • Author(s)
      望月早月
    • Organizer
      第73回日本癌学会学術総会
    • Place of Presentation
      横浜市、神奈川県、パシフィコ横浜
    • Year and Date
      2014-09-25 – 2014-09-27
    • Related Report
      2014 Annual Research Report
  • [Presentation] メタロプロテアーゼADAM28を標的としたヒト型活性阻害抗体の開発2014

    • Author(s)
      望月早月
    • Organizer
      第19回日本病態プロテアーゼ学会学術集会
    • Place of Presentation
      豊中市、大阪府、千里ライフサイエンスセンター
    • Year and Date
      2014-08-08 – 2014-08-09
    • Related Report
      2014 Annual Research Report
  • [Presentation] 非小細胞肺癌の分子標的治療を目指したヒト型抗ADAM28抗体の作用機構2014

    • Author(s)
      望月早月
    • Organizer
      第23回日本がん転移学会学術集会・総会
    • Place of Presentation
      金沢市、石川県、金沢市文化ホール
    • Year and Date
      2014-07-10 – 2014-07-11
    • Related Report
      2014 Annual Research Report
  • [Presentation] Effects of human antibodies against ADAM28 on cancer cell growth and metastasis2014

    • Author(s)
      Satsuki Mochizuki
    • Organizer
      1st Matrix Biology Europe Conference
    • Place of Presentation
      Rotterdam、オランダ、De Doelen Concert and Congress Center
    • Year and Date
      2014-06-21 – 2014-06-24
    • Related Report
      2014 Annual Research Report
  • [Presentation] Selective inhibition of ADAM28 activity by human anti-ADAM28 antibodies suppresses cancer cell proliferation and metastasis2013

    • Author(s)
      Satsuki Mochizuki, Hitoshi Abe, Masyuki Shimoda, Noriko Aramaki-Hattori, Yuka Miyamae, Akira Miyakoshi, Kanehisa Kojoh and Yasunori Okada
    • Organizer
      9th Pan Pacific Connective Tissue Societies Symposium
    • Place of Presentation
      Hong Kong
    • Related Report
      2013 Research-status Report
  • [Presentation] SrcはADAM28の発現に重要な役割を果たす2013

    • Author(s)
      望月早月、阿部仁、尾原健太郎、落合大樹、北川雄光、樋野興夫、佐藤博、 岡田保典
    • Organizer
      第72回日本癌学会学術総会
    • Place of Presentation
      横浜
    • Related Report
      2013 Research-status Report
  • [Presentation] メタロプロテアーゼADAM28の腫瘍細胞における発現機構と悪性化への関与2013

    • Author(s)
      望月早月、阿部仁、尾原健太郎、上野万里、落合大樹、北川雄光、樋野興夫、佐藤博、岡田保典
    • Organizer
      第18回日本病態プロテアーゼ学会学術集会
    • Place of Presentation
      大阪
    • Related Report
      2013 Research-status Report
  • [Presentation] ヒトADAM28活性阻害抗体の開発とそれによる癌細胞増殖・転移抑制作用2013

    • Author(s)
      望月早月、岡田保典
    • Organizer
      第22回日本がん転移学会学術集会・総会
    • Place of Presentation
      松本
    • Related Report
      2013 Research-status Report
  • [Presentation] ADAM28による癌細胞転移機構とその制御2013

    • Author(s)
      望月早月、下田将之、宮越陽、古城周久、岡田保典
    • Organizer
      第45回日本結合組織学会学術大会、第60回マトリックス研究会大会合同学術大会
    • Place of Presentation
      和歌山
    • Related Report
      2013 Research-status Report
  • [Presentation] ADAM28 as a possible molecular target for non-small cell lung carcinoma (NSCLC) and breast carcinoma2012

    • Author(s)
      Satsuki Mochizuki, Masayuki Shimoda and Yasunori Okada
    • Organizer
      第71回日本癌学会学術総会
    • Place of Presentation
      札幌
    • Related Report
      2012 Research-status Report
  • [Presentation] Novel effect of ADAM28 on promotion of carcinoma cell metastasis2012

    • Author(s)
      Satsuki Mochizuki, Kenji Soejima, Masayuki Shimoda, and Yasunori Okada
    • Organizer
      XXIIIrd FECTS and ISMB Joint Meeting
    • Place of Presentation
      Katowice
    • Related Report
      2012 Research-status Report
  • [Presentation] メタロプロテアーゼADAM28によるvon Willebrand factor分解と癌細胞転移機構2012

    • Author(s)
      望月早月、副島見事、下田将之、阿部仁、佐々木文、岡野ジェイムズ洋尚、岡野栄之、岡田保典
    • Organizer
      第17回日本病態プロテアーゼ学会学術集会
    • Place of Presentation
      浜松
    • Related Report
      2012 Research-status Report
  • [Presentation] ADAM28による癌細胞新規転移促進機構2012

    • Author(s)
      望月早月、副島見事、下田将之、岡田保典
    • Organizer
      第21回日本がん転移学会学術集会・総会
    • Place of Presentation
      広島
    • Related Report
      2012 Research-status Report
  • [Presentation] ADAM28のvon Willebrand factor分解による新規癌細胞転移機構2012

    • Author(s)
      望月早月、副島見事、下田将之、阿部仁、佐々木文、岡野ジェイムズ洋尚、岡野英之、岡田保典
    • Organizer
      第101回日本病理学会総会
    • Place of Presentation
      東京
    • Related Report
      2012 Research-status Report
  • [Book] Handbook of Proteolytic Enzymes2013

    • Author(s)
      Satsuki Mochizuki and Yasunori Okada
    • Publisher
      Elsevier Ltd
    • Related Report
      2013 Research-status Report
  • [Book] Handbook of Proteolytic Enzymes2013

    • Author(s)
      Satsuki Mochizuki and Yasunori Okada
    • Total Pages
      4094
    • Publisher
      Academic Press
    • Related Report
      2012 Research-status Report
  • [Remarks] 慶應義塾大学医学部岡田研究室

    • URL

      http://keio-okada-lab.jp/

    • Related Report
      2014 Annual Research Report
  • [Remarks] 慶應義塾大学医学部 岡田研究室

    • URL

      http://keio-okada-lab.jp/

    • Related Report
      2013 Research-status Report

URL: 

Published: 2013-05-31   Modified: 2019-07-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi