Analysis of gene expression on interstitial cells of Cajal

• Project/Area Number: 24590494
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Experimental pathology
• Research Institution: Hyogo Medical University
• Principal Investigator: ISOZAKI Koji 兵庫医科大学, 医学部, 非常勤講師 (00425117)
• Co-Investigator(Kenkyū-buntansha): HIROTA Seiichi 兵庫医科大学, 医学部, 教授 (50218856)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: カハールの介在細胞 / c-kit / GISTs / CADM1 / c-kit遺伝子 / c-kit 遺伝子

Outline of Final Research Achievements

By using cDNA library of interstitial cells of Cajal(ICCs), we first clarified that CADM1, one of cell adhesion molecules, is specifically expressed by ICCs. We investigated CADM1 expression in gastrointestinal stromal tumors (GISTs) which are considered to be originated from ICCs, and found that most of GISTs in the small intestine express CADM1, but most of gastric GISTs do not. The specific expression of CADM1 in small intestinal GISTs might mean that specific expression of CADM1 in ICCs in the small intestine. ICCs in different sites may show different protein expression pattern and may have different role.

Research Products

• [Journal Article] Gastrointestinal stromal tumors with exon8 c-kit gene mutation might occur at extragastric sites and have metastasis-prone nature (2014)
  • Author(s): Ito T,Yamamura M,Hirai T,Ishikawa T,Kanda T,Nakai T,Ohkouchi M,Hashikura Y,Isozaki K,Hirota S
  • Journal Title: In J Clin Exp Pathology Volume: 7 Pages: 80248031-80248031
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Extracellular domain c-kit mutation with duplication of Ser501Ala502 found in gastrointestinal stromal tumors is more imatinib- and nilotinib-sensitive than that with duplication of Ala502Tyr503 (2013)
  • Author(s): Liu N-N, Ohkouchi M, Hashikura Y, Kajimoto N, Matsuda I, Isozaki K, Toh Y, Takahashi T, Nishida T, Hirota S
  • Journal Title: Lab Invest Volume: 93 Issue: 5 Pages: 502-507
  • DOI: 10.1038/labinvest.2013.43
  • Peer Reviewed
• [Journal Article] Characterization of novel germline c-kit gene mutation, KIT-Tyr553Cys, observed in a family with multiple gastrointestinal stromal tumors (2012)
  • Author(s): Nakai M, Hashikura Y, Ohkouchi M, Yamamura M, Akiyama T, Shiba K, Kajimoto N, Tsukamoto Y, Hao H, Isozaki K, Hirai T, Hirota S
  • Journal Title: Lab Invest Volume: 92 Issue: 3 Pages: 451-457
  • DOI: 10.1038/labinvest.2011.165
  • Peer Reviewed
• [Presentation] c-kit遺伝子のexon8に変異を持つ消化管間葉系腫瘍にはimatinibによる分子標的治療が有効 (2015)
  • Author(s): 礒崎耕次
  • Organizer: 第１６回関西がんチーム医療研究会
  • Place of Presentation: 大阪
  • Year and Date: 2015-02-28