Comprehensive study on the mechanisms involved in micropapillary or budding type tumor invasion

• Project/Area Number: 24590495
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Experimental pathology
• Research Institution: Fukuoka University
• Principal Investigator: Nabeshima Kazuki 福岡大学, 医学部, 教授 (40189189)
• Co-Investigator(Kenkyū-buntansha): Koga Kaori 福岡大学, 医学部, 助教 (40572433) ; Aoki Mikiko 福岡大学, 医学部, 講師 (80469379) ; Hamasaki Makoto 福岡大学, 医学部, 講師 (90412600)
• Co-Investigator(Renkei-kenkyūsha): Tsunoda Toshiyuki 福岡大学, 医学部, 准教授 (70444817)
• Project Period (FY): 2012-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2012: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
• Keywords: 簇出型浸潤 / miRNA / 肺癌 / micropapillary pattern / tumor budding / sprouting / 大腸癌 / 頭頸部癌 / Micropapillary pattern / microdissection

Outline of Final Research Achievements

The micropapillary pattern (MPP) observed in lung adenocarcinoma is a poor prognostic factor. We previously reported that MPP-positive adenocarcinoma were significantly associated with presence of small cluster invasion, which is similar to tumor budding at the invasion front of colon adenocarcinoma. To elucidate the mechanisms in such tumor budding type tumor cell invasion is the purpose of this study. By microdissection, extraction of RNA, and miRNA array performed in human lung adenocarcinoma tissue sections with or without MPP, we identified hsa-miR-663b, which was significantly upregulated in MPP-positive carcinomas. Localization of the miRNA signals was shown by in situ hybridization using Locked RNA.

Research Products

• [Journal Article] Tumor budding and laminin 5γ2 in squamous cell carcinoma of the external auditory canal are associated with shorter survival. (2015)
  • Author(s): Okado Y, Aoki M, Hamasaki M, Koga K, Sueta T, Shiratsuchi H, Oda Y, Nakagawa T, Nabeshima K.
  • Journal Title: SpringerPlus Volume: ４ Issue: 1
  • DOI: 10.1186/s40064-015-1620-4
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Tumor budding in colorectal carcinoma assessed by cytokeratin immunostaining and budding areas: Possible involvement of c-Met. (2014)
  • Author(s): Satoh K, Nimura S, Hamasaki M, Aoki M, Koga K, Iwasaki H, Yamashita Y, Kataoka H, Nabeshima K
  • Journal Title: Cancer Science Volume: 105 Issue: 11 Pages: 1487-1495
  • DOI: 10.1111/cas.12530
  • Peer Reviewed / Open Access
• [Journal Article] Association of c-Met phosphorylation with micropapillary pattern and small cluster invasion in pT1-size lung adenocarcinoma. (2013)
  • Author(s): Koga, K., Hamasaki, M., Kato, F. Aoki, M., Hayashi, H., Iwasaki, A., Kataoka, H., Nabeshima, K.
  • Journal Title: Lung Cancer Volume: 82 Pages: 413-419
  • Peer Reviewed
• [Presentation] 外耳道扁平上皮癌におけるtumor buddingおよびlaminin5-γ2の発現と予後との関連. (2015)
  • Author(s): 宮崎健、大門康子、青木光希子、濵﨑慎、末田尚之、中川尚志、鍋島一樹.
  • Organizer: 第47回日本結合組織学会学術大会
  • Place of Presentation: 東京（品川コクヨホール）
  • Year and Date: 2015-05-15
• [Presentation] 大腸癌tumor budding評価におけるサイトケラチン免疫染色の有用性とcMetの関連について. (2013)
  • Author(s): 佐藤啓介、二村聡、濵﨑慎、青木光希子、古賀佳織、林博之、山下裕一、鍋島一樹.
  • Organizer: 第102回日本病理学会総会
  • Place of Presentation: 札幌
• [Presentation] pT1肺腺癌(≦30mm)の腫瘍サイズによる浸潤形態の比較検討 -特にMicropapillary predominant の意義について- (2012)
  • Author(s): 加藤文章、濱崎慎、林博之、岩崎昭憲、鍋島一樹.
  • Organizer: 第53回日本肺癌学会総会
  • Place of Presentation: 岡山