| Project/Area Number |
24590498
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Experimental pathology
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| Research Institution | National Institute of Advanced Industrial Science and Technology |
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Principal Investigator |
IKEHARA YUZURU 独立行政法人産業技術総合研究所, バイオメデイカル研究部門, 上級主任研究員 (10311440)
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| Co-Investigator(Renkei-kenkyūsha) |
YAMAGUCHI Takashi 独立行政法人産業技術総合研究所, バイオメデイカル研究部門, 契約職員 (60626563)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
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| Keywords | 膵臓がん / がん幹細胞 / 細胞分化 / 免疫治療 / 腺管構造の形成 / 幹細胞Sphere / 免疫応答 / T抗原エピトープ / マウスモデル / 三次元培養 / 不死化 / 幹細胞 / 腫瘍 |
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| Outline of Final Research Achievements |
This study was conducted to increase knowledge about nature of stem cells in CK19+ pancreatic ductal epithelial cells. Established mouse pancreatic cancer and immortalized cell lines with CK19+ formed sphere structure (SS) and tubular structure (TS) in three dimension culture using collagen gels. We have demonstrated that there were differential types of stem cells in SS and TS, and presence of the cells determines SS and TS, respectively. Additionally, TGFβ and the signal transduction pathway was identified to promote from SS to TS conversion. Furthermore, immune reaction could control cancer cells growth of both SS and TS, while rejection of tumor cells was impaired by tolerance linked with CD4+CD25+regulatory T cells and monocyte with CD11b+ /F4/80+.
In conclusion, though ｔhere are different types of stem cells in pancreatic cancer, immune therapy is feasible to use for control over the differentiation status.
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