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Generation of rodent malaria parasite with high mutation rate and confirmation of its usefulness for next generation of malariology

Research Project

Project/Area Number 24590501
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Parasitology (including Sanitary zoology)
Research InstitutionJuntendo University (2014-2015)
Gunma University (2012-2013)

Principal Investigator

Makoto Hirai  順天堂大学, 医学部, 准教授 (50326849)

Project Period (FY) 2012-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywordsmutator malaria / ネズミマラリア原虫 / ミューテーター / 熱帯熱マラリア原虫
Outline of Final Research Achievements

We have succeeded to generate mutator malaria parasite, Plasmodium berghei with destructed proof reading activity in DNA polymerase delta. We confirmed that the mutator malaria parasite shows 80 times higher mutation rate than wild-type parasite. Moreover, we succeeded to isolate several drug resistant parasites from mutator malaria parasite, which could be utilized for further research to reveal molecular mechanisms of parasite drug resistance. A series of these results provide the proof that our mutator parasite could be suitable research tool for parasite biology, especially drug resistance.

Report

(5 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • 2013 Research-status Report
  • 2012 Research-status Report
  • Research Products

    (21 results)

All 2016 2015 2014 2013 2012 Other

All Journal Article (15 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 15 results,  Open Access: 6 results,  Acknowledgement Compliant: 2 results) Presentation (3 results) (of which Invited: 1 results) Book (2 results) Remarks (1 results)

  • [Journal Article] Plasmodium falciparum kelch 13: a potential molecular marker for tackling artemisinin-resistant malaria parasites.2016

    • Author(s)
      Mita T, Tachibana S, Hashimoto M, Hirai M.
    • Journal Title

      Expert Rev Anti Infect Ther.

      Volume: 14 Issue: 1 Pages: 125-135

    • DOI

      10.1586/14787210.2016.1106938

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Visualization of Malaria Parasites in the Skin Using the Luciferase Transgenic Parasite,<i> Plasmodium berghei</i>2015

    • Author(s)
      Matsuoka H, Tomita H, Hattori R, Arai M, Hirai M.
    • Journal Title

      Tropical Medicine and Health

      Volume: 43 Issue: 1 Pages: 53-61

    • DOI

      10.2149/tmh.2014-18

    • NAID

      130004704899

    • ISSN
      1348-8945, 1349-4147
    • Related Report
      2015 Annual Research Report 2014 Research-status Report
    • Peer Reviewed
  • [Journal Article] Direct evidence for the atovaquone action on the Plasmodium cytochrome bc1 complex.2015

    • Author(s)
      Siregar JE, Kurisu G, Kobayashi T, Matsuzaki M, Sakamoto K, Mi-Ichi F, Watanabe Y, Hirai M, Matsuoka H, Syafruddin D, Marzuki S, Kita K.
    • Journal Title

      Parasitol Int

      Volume: 64 Issue: 3 Pages: 295-300

    • DOI

      10.1016/j.parint.2014.09.011

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Lactate retards the development of erythrocytic stages of the human malaria parasite Plasmodium falciparum.2015

    • Author(s)
      Hikosaka K, Hirai M, Komatsuya K, Ono Y, Kita K.
    • Journal Title

      Parasitol Int

      Volume: 64 Issue: 3 Pages: 301-303

    • DOI

      10.1016/j.parint.2014.08.003

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Plasmodium berghei ANKA causes intestinal malaria associated with dysbiosis2015

    • Author(s)
      Taniguchi T, Miyauchi E, Nakamura S, Hirai M, Suzue K, Imai T, Nomura T, Handa T, Okada H, Shimokawa C, Onishi R, Olia A, Hirata J, Tomita H, Ohno H, Horii T, Hisaeda H
    • Journal Title

      Scientific Reports

      Volume: 5:15699 Issue: 1 Pages: 1-12

    • DOI

      10.1038/srep15699

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Evaluating experimental cerebral malaria using oxidative stress indicator OKD48 mice.2014

    • Author(s)
      Imai T, Iwawaki T, Akai R, Suzue K, Hirai M, Taniguchi T, Okada H, Hisaeda H.
    • Journal Title

      Int J Parasitol.

      Volume: 44 Issue: 10 Pages: 681-685

    • DOI

      10.1016/j.ijpara.2014.06.002

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Generation of rodent malaria parasites with a high mutation rate by destructing proofreading activity of DNA polymerase δ.2014

    • Author(s)
      Honma H, Hirai M, Nakamura S, Hakimi H, Kawazu S, Palacpac NM, Hisaeda H, Matsuoka H, Kawai S, Endo H, Yasunaga T, Ohashi J, Mita T, Horii T, Furusawa M, Tanabe K.
    • Journal Title

      DNA Res

      Volume: 21 Issue: 4 Pages: 439-46

    • DOI

      10.1093/dnares/dsu009

    • Related Report
      2014 Research-status Report 2013 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Species-specific immunity induced by infection with Entamoeba histolytica and Entamoeba moshkovskii in mice2013

    • Author(s)
      Shimokawa, C., Culleton, R., Imai, T., Suzue, K., Hirai, M., Taniguchi, T., Kobayashi, S., Hisaeda, H., Hamano, S
    • Journal Title

      PLoS One

      Volume: 8(11) Issue: 11 Pages: e82025-e82025

    • DOI

      10.1371/journal.pone.0082025

    • NAID

      120006986684

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] IL-23 protection against Plasmodium berghei infection in mice is partially dependent on IL-17 from macrophages2013

    • Author(s)
      Ishida H, Imai T, Suzue K, Hirai M, Taniguchi T, Yoshimura A, Iwakura Y, Okada H, Suzuki T, Shimokawa C, and Hisaeda
    • Journal Title

      Eur. J. Immunol.

      Volume: 43 Issue: 10 Pages: 2696-2706

    • DOI

      10.1002/eji.201343493

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Characterazation of burdock mottle virus, a novel member of the genus Benyvirus, and the presence of benyvirus-related sequences in the plant and insect genomes.2013

    • Author(s)
      Kondo, H. , Hirano, S., Chiba, S., Andika, I.B., Hirai, M., Maeda, T. and Tamada, T.
    • Journal Title

      Virus Research

      Volume: 177 Issue: 1 Pages: 75-86

    • DOI

      10.1016/j.virusres.2013.07.015

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] CD8(+) T cell activation by murine erythroblasts infected with malaria parasites.2013

    • Author(s)
      Imai T, Ishida H, Suzue K, Hirai M, Taniguchi T, Okada H, Suzuki T, Shimokawa C, Hisaeda H.
    • Journal Title

      Sci Rep.

      Volume: 3 Issue: 1 Pages: 1572-1572

    • DOI

      10.1038/srep01572

    • NAID

      120006985954

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Resistance to Malaria by Enhanced Phagocytosis of Erythrocytes in LMP7-deficient Mice.2013

    • Author(s)
      Duan X, Imai T, Chou B, Tu L, Himeno K, Suzue K, Hirai M, Taniguchi T, Okada H, Shimokawa C, Hisaeda H.
    • Journal Title

      PLoS One.

      Volume: 8 Issue: 3 Pages: 1-9

    • DOI

      10.1371/journal.pone.0059633

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] One Injection of DsRed Followed by Bites from Transgenic Mosquitoes Producing DsRed in the Saliva Elicits a High Titer of Antibody in Mice2012

    • Author(s)
      Matsuoka H, Sano G, Hattori R, Tomita H, Yamamoto DS, Hirai M.
    • Journal Title

      Tropical Medicine and Health

      Volume: 40 Issue: 2 Pages: 47-53

    • DOI

      10.2149/tmh.2011-10

    • NAID

      10031123157

    • ISSN
      1348-8945, 1349-4147
    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Critical roles of the mitochondrial complex II in oocyst formation of rodent malaria parasite Plasmodium berghei.2012

    • Author(s)
      Hino A, Hirai M, Tanaka TQ, Watanabe Y, Matsuoka H, Kita K.
    • Journal Title

      J Biochem

      Volume: 152 Issue: 3 Pages: 259-68

    • DOI

      10.1093/jb/mvs058

    • NAID

      10031166137

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Toward understanding the role of mitochondrial complex II in the intraerythrocytic stages of Plasmodium falciparum: gene targeting of the Fp subunit.2012

    • Author(s)
      Tanaka TQ, Hirai M, Watanabe Y, Kita K.
    • Journal Title

      Parasitol Int

      Volume: 61 Issue: 4 Pages: 726-8

    • DOI

      10.1016/j.parint.2012.06.002

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Presentation] 比較トランスクリプトームによるマラリア原虫受精関連因子の探索2014

    • Author(s)
      平井 誠、新井 明治 、安彦 真文、本間 一、岡本 龍史、鈴江 一友、今井 孝、谷口 委代、堀井 俊宏、田邉 和裄、久枝 一
    • Organizer
      日本寄生虫学会
    • Place of Presentation
      愛媛県松山市
    • Related Report
      2013 Research-status Report
  • [Presentation] 比較トランスクリプトームによるマラリア原虫受精因子の探索2012

    • Author(s)
      平井誠、安彦真文、岡本龍司、本間一、鈴江一友、今井孝、堀井俊宏、田邉和裄、久枝一
    • Organizer
      第10回分子寄生虫・マラリアフォーラム、第72回日本寄生虫学会東日本支部会合同大会
    • Place of Presentation
      群馬大学医学部
    • Related Report
      2012 Research-status Report
  • [Presentation] マラリア原虫をやっつける~その驚異、生態、新たな対策2012

    • Author(s)
      平井 誠
    • Organizer
      第2回 WIAS Top Runners’ Lecture Collection of Science
    • Place of Presentation
      早稲田大学
    • Related Report
      2012 Research-status Report
    • Invited
  • [Book] 生物の受精2014

    • Author(s)
      森稔幸、平井 誠
    • Publisher
      化学と生物
    • Related Report
      2013 Research-status Report
  • [Book] Sexual reproduction2014

    • Author(s)
      Makoto Hirai
    • Publisher
      Elsevier
    • Related Report
      2013 Research-status Report
  • [Remarks] マラリア生物学研究のための新しいツールを開発―マラリア撲滅のための道を拓くー

    • URL

      http://www.gunma-u.ac.jp/sb/sb.cgi?eid=829

    • Related Report
      2013 Research-status Report

URL: 

Published: 2013-05-31   Modified: 2019-07-29  

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