| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Outline of Final Research Achievements |
In this study, we demonstrated that PyGM75 in Plasmodium yoelii is localized to the osmiophilic bodies in the cytoplasm of male gametocyte then released to the surface of microgamete by immune electron microscopy using specific antibodies against PyGM75. By gene disruption strategy, it was shown that PyGM75 plays a crucial role during exflagellation after ingestion by mosquito. In addition, to examine whether anti-PyGM75 antibodies can prevent parasite transmission to mosquito, we mixed the specific antibodies and parasitized RBCs, then let them feed on Anopheles mosquitoes using membrane-feeding system. As a result, the numbers of oocysts formed on the mosquito midgut were greatly reduced by adding anti-PyGM75 antibodies. This result strongly suggests that GM75 is a novel candidate target of transmission-blocking vaccine.
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