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Ultrastructural analysis of Maurer's clefts of Plasmodium falciparum infected red blood cell

Research Project

Project/Area Number 24590508
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Parasitology (including Sanitary zoology)
Research InstitutionNagasaki University

Principal Investigator

SAKAGUCHI Miako  長崎大学, 熱帯医学研究所, 助教 (50400651)

Co-Investigator(Kenkyū-buntansha) YAHATA Kazuhide  長崎大学, 熱帯医学研究所, 助教 (40467965)
Co-Investigator(Renkei-kenkyūsha) KANEKO Osamu  長崎大学, 熱帯医学研究所, 教授 (50325370)
Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywordsマラリア / 感染赤血球 / 免疫染色 / 電子顕微鏡 / マウレル裂
Outline of Final Research Achievements

To clarify the formation process and ultrastructure of Maurer’s clefts in the Plasmodium falciparum infected red blood cell, we generated transgenic P. falciparum lines expressing Pfmc2TM fused with GFP and several epitope tags such as Flag or Myc. We found that the protein localized to the Maurer’s clefts. However, we could not use the transgenic lines for live imaging by confocal microscopy, since the GFP signal was very low intensity. Next, we used SBF-SEM to image the 3D structure of multiple entire P. falciparum infected red blood cells at each stages. The 3D organization showed that Maurer’s clefts are not connected to the parasitophorous vacuole, red blood cell membrane, or tubovesiclar network. They are not continuous membrane networks but independent membranous structures in the red blood cell.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • 2012 Research-status Report
  • Research Products

    (10 results)

All 2015 2014 2012

All Journal Article (2 results) (of which Peer Reviewed: 2 results,  Open Access: 1 results,  Acknowledgement Compliant: 1 results) Presentation (8 results) (of which Invited: 1 results)

  • [Journal Article] Expression and localization of rhoptry neck protein 5 in merozoites and sporozoites of Plasmodium yoelii.2014

    • Author(s)
      Mutungi JK, Yahata K, Sakaguchi M, *Kaneko O.
    • Journal Title

      Parasitol Int

      Volume: 63 Issue: 6 Pages: 794-801

    • DOI

      10.1016/j.parint.2014.07.013

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Tanay virus, a new species of virus isolated from mosquitoes in the Philippines2014

    • Author(s)
      T. Nabeshima, S. Inoue, K. Okamoto, P.H. Guillermo, F. Yu, L. Uchida, A. Ichinose, M. Sakaguchi, T. Sunahara, C.C. Buerano, F.P. Tadena, I.B. Orbita, F.F. Natividad, and K. Morita
    • Journal Title

      Journal of General Virology

      Volume: - Issue: 6 Pages: 1390-1395

    • DOI

      10.1099/vir.0.061887-0

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Presentation] SBF-SEMによるマラリア感染赤血球の三次元観察とストラクトーム解析2015

    • Author(s)
      坂口美亜子、宮崎直幸、藤岡壽、金子修、村田和義
    • Organizer
      日本寄生虫学会
    • Place of Presentation
      杏林大学(東京都・三鷹市)
    • Year and Date
      2015-03-21 – 2015-03-22
    • Related Report
      2014 Annual Research Report
  • [Presentation] SBF-SEMによるマラリア感染赤血球の三次元構造解析2015

    • Author(s)
      坂口美亜子、宮崎直幸、藤岡壽、金子修、村田和義
    • Organizer
      日本顕微鏡学会関東支部講演会
    • Place of Presentation
      工学院大学(東京都・新宿区)
    • Year and Date
      2015-02-28
    • Related Report
      2014 Annual Research Report
  • [Presentation] SBF-SEMを用いたマラリア感染赤血球の三次元観察とストラクトーム解析2014

    • Author(s)
      坂口美亜子、宮崎直幸、藤岡壽、金子修、村田和義
    • Organizer
      生理研研究会
    • Place of Presentation
      岡崎コンファレンスセンター(愛知県・岡崎市)
    • Year and Date
      2014-11-12 – 2014-11-13
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] 3D structural analysis of malaria parasite-infected red blood cells by SBF-SEM2014

    • Author(s)
      Miako Sakaguchi, Naoyuki Miyazaki, Hisashi Fujioka, Osamu Kaneko, Kazuyoshi Murata
    • Organizer
      International Microscopy Congress
    • Place of Presentation
      Praque Congress Centre(プラハ(チェコ))
    • Year and Date
      2014-09-07 – 2014-09-12
    • Related Report
      2014 Annual Research Report
  • [Presentation] SBF-SEMを用いたマラリア感染赤血球の三次元構造解析2014

    • Author(s)
      坂口美亜子、宮崎直幸、藤岡壽、金子修、村田和義
    • Organizer
      日本医学生物学電子顕微鏡技術学会
    • Place of Presentation
      大阪大学(大阪府・吹田市)
    • Year and Date
      2014-05-23 – 2014-05-25
    • Related Report
      2014 Annual Research Report
  • [Presentation] SBF-SEMによるマラリア感染赤血球の三次元観察2014

    • Author(s)
      坂口美亜子、宮崎直幸、藤岡壽、金子修、村田和義
    • Organizer
      日本顕微鏡学会
    • Place of Presentation
      幕張メッセ(千葉県・千葉市)
    • Year and Date
      2014-05-11 – 2014-05-13
    • Related Report
      2014 Annual Research Report
  • [Presentation] Localization of Plasmodium falciparum antigen 332, a gigantic protein expressed in the parasite-infected red blood cell2012

    • Author(s)
      坂口美亜子
    • Organizer
      マトリョーシカ型生物学研究会
    • Place of Presentation
      東京(国立感染症研究所)
    • Related Report
      2012 Research-status Report
  • [Presentation] Role of the C-terminal region of Plasmodium falciparum antigen 332 on the location in the parasite-infected red blood cell2012

    • Author(s)
      坂口美亜子
    • Organizer
      Molecular Parasitology Meeting
    • Place of Presentation
      Woods hole, USA
    • Related Report
      2012 Research-status Report

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Published: 2013-05-31   Modified: 2019-07-29  

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