Application of cell-free schistosome DNA to a diagnostic for active schistosomiases
Project/Area Number |
24590510
|
Research Category |
Grant-in-Aid for Scientific Research (C)
|
Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Parasitology (including Sanitary zoology)
|
Research Institution | Dokkyo Medical University |
Principal Investigator |
|
Co-Investigator(Kenkyū-buntansha) |
CHIGUSA Yuichi 獨協医科大学, 医学部, 教授 (20171936)
|
Project Period (FY) |
2012-04-01 – 2015-03-31
|
Project Status |
Completed (Fiscal Year 2014)
|
Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2012: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
|
Keywords | 住血吸虫症 / 活動性感染 / 検査・診断 / 遊離型DNA / 診断 / 診断法 |
Outline of Final Research Achievements |
Cell-free schistosome DNA is fragments of parasite-derived DNA exists in the host’s bodily fluids (blood, urine, and saliva). We evaluated the usefulness of cell-free schistosome DNA as a diagnostic marker for active schistosomiases. In schiatoomiasis-endemic areas in the Philippines, where the annual mass deworming program has been implemented, the active schistosomiasis detected by cell-free schistosome DNA was 3.5 to 10 times higher than that by stool examination (gold standard). In the case of refractory imported schistosomiasis haematobia, parasite egg rapidly disappeared after anthelmintic administration, whereas, cell-free schistosome DNA remained persistent. This was consistent with the presence of morphologically intact ova in bladder biopsy samples and with the corresponding symptoms. These results suggest that cell-free schistosome DNA, independent for parasite egg, is a promising diagnostic marker for active schistosomiases.
|
Report
(4 results)
Research Products
(11 results)
-
-
[Journal Article] Detection of active schistosome infection by cell-free circulating DNA of Schistosoma japonicum in highly endemic areas in Sorsogon Province, the Philippines2014
Author(s)
Kato-Hayashi N, Leonardo LR, Arevalo NL, Tagum MNB, Apin J, Agsolid LM, Chua JC, Villacorte EA, Kirinoki M, Kikuchi M, Ohmae H, Haruki K, Chigusa Y
-
Journal Title
Acta Tropica
Volume: 141
Pages: 178-183
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
-
[Journal Article] Use of cell-free circulating schistosome DNA in serum, urine, semen, and saliva to monitor a case of refractory imported schistosomiasis hematobia2013
Author(s)
Kato-Hayashi N, Yasuda M, Yuasa J, Isaka S, Haruki K, Ohmae H, Osada Y, Kanazawa T, Chigusa Y
-
Journal Title
J Clin Microbiol
Volume: 51(10)
Issue: 10
Pages: 3435-8
DOI
Related Report
Peer Reviewed
-
-
-
-
[Presentation] 尿中の住血吸虫遊離型DNAを標的とした活動性感染の検出2013
Author(s)
林尚子, James C. Chua, Lydia R. Leonardo, Napoleon L. Arevalo, Ma. Nerissa B. Tagum, Lea Agsorid, 菊池三穂子、大前比呂思、春木宏介、千種雄一
Organizer
第82回日本寄生虫学会大会
Place of Presentation
東京医科歯科大学 湯島キャンパス
Related Report
-
[Presentation] A clinical trial for detection of active schistosome infection by cell-free circulating schistosome DNA in chronic patients in the schistosomiasis laponica highly endemic area in the Philippines.2012
Author(s)
Hiroshi Ohmae, Naoko Kato-Hayashi, Lydia R. Leonardo, Napoleon L. Arevalo, Ma. Nerissa B. Tagum, Lea Agsorid, Masashi Kirinoki, Mihoko Kikuchi, Kousuke Haruki, Yuichi Chigusa
Organizer
The 12th Annual Workshop of the Regional Network on Asian Schistosomiasis and Other Healminth Zoonosis (RNAS+) Hanoi, Vietnam
Place of Presentation
MOD Palace Hotel, Hanoi, Vietnam
Related Report
-
-
-