| Project/Area Number |
24590512
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Parasitology (including Sanitary zoology)
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| Research Institution | Teikyo University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
ONO Yasuo 帝京大学, 医学部, 教授 (10177272)
UBAGAI Tsuneyuki 帝京大学, 医学部, 講師 (10311416)
KIKUCHI Hirotoshi 帝京大学, 医学部, 准教授 (80338681)
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| Project Period (FY) |
2012-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 原虫 / 免疫調節 / 細胞外ヌクレオチド酵素 / immunomodulator / 遺伝子発現 / inflammatory response |
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| Outline of Final Research Achievements |
Immuno-modulate effects of T. gondii NTPase on neutrophils or mast cells were examined in this research project. When neutrophils or mast cells were exposed with ATP in vitro, gene expressions of TNF-α and IL-8 were down-regulated. However, presence of NTPase with ATP neutralized those effects of down-regulation. Gene expression of CD39 that is as known as ecto ATPase in neutrophil were also decreased. Neutrophils exposed both of ATP and NTPase showed that the equal CD39 expression with control. The mRNA expression of an adenosine receptor, AdoRA2a was down-regulated by NTPase. No effects of ATP or NTPase were observed in CD73 expression. It is suggested that T. gondii might use NTPase as an immune-modulator to escape from neutrophils or other immune cells in infection.
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