| Project/Area Number |
24590526
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Bacteriology (including Mycology)
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| Research Institution | Kagawa University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
KUWAHARA Tomomi 香川大学, 医学部・分子微生物学, 教授 (60263810)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | Bacteroides fragilis / 莢膜 / 相変異 / DNA逆位 |
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| Outline of Final Research Achievements |
The human gut microbe Bacteroides fragilis can alter the expression of its surface molecules such as capsular polysaccharides (PS) through the reversible DNA inversions. B. fragilis strain YCH46 possesses master DNA invertases, Mpi that mediates the on-off switching of seven promoter regions for PS biosynthesis. However the underlying molecular mechanisms to regulate the DNA inversions by Mpi are still unclear. In this study, we identified the thirteen Mpi-binding proteins by in vitro pull-down assay. The mpi gene colocalizes with a tyrosine-type site-specific recombinase gene (BF2766) in a head-to-head manner. By comparing the transcriptomes of BF2766 deletion mutants, we found that BF2766-mediated inversion controlled the expression of Mpi as well, which in turn regulated the expression of PS loci in a hierarchical manner, allowing a coordinate alteration of surface structures.
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